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Using Bioluminescent Reporter Genes to Optimize shRNA Target Sites for RNAi of the bcr/abl Gene

Natalie Betz
Promega Corporation
Publication Date: 2005

Abstract

Not all shRNAs are equally effective at suppressing target gene expression in mammalian cells. Therefore it is important to quickly and easily identify the shRNA sequence that is the most effective at inducing RNA interference for a particular target gene sequence. The psiCHECK™-2 Vector, which contains two luminescent reporter genes, was used to identify the most effective shRNA for inducing RNAi of the bcr/abl gene. The seven shRNAs tested were generated using the T7 RiboMAX™ Express RNAi System.

Promega Notes 90, 26–28.