Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

siCHECK™ Vectors

Instructions for Use of Product(s)

Literature # TB329

The psiCHECK™-1 Vector and psiCHECK™-2 Vector provide a quantitative and rapid approach to optimize RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to the reporter gene. In both vectors, Renilla luciferase is used as a primary reporter gene, and the gene of interest can be cloned into the multiple cloning region located downstream of the Renilla translational stop codon. Initiation of the RNAi process towards a gene of interest results in cleavage and subsequent degradation of fusion mRNA. Measurement of decreased Renilla luciferase activity is a convenient indicator of RNAi effect.

Summary of Changes
The following changes were made to the 4/16 revision of this document:
1. Removed discussion of using this product with shRNA.

Printed in USA. Revised 4/16.