Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Measuring the QuantiFluor™ dsDNA System Using the QuantiFluor™-ST Fluorometer

Publication Date: 11/12

Abstract

Traditional spectrophotometric assays cannot determine DNA concentration below 2µg/ml; however, many isolated DNA samples have concentrations well below that level. The QuantiFluor™ dsDNA System provides a fast, easy and sensitive method for determining DNA concentrations as low as 2.5ng/ml (or 0.5ng/well). The QuantiFluor™ dsDNA System contains a fluorescent DNA-binding dye that enables sensitive and specific quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.

This Application Note describes the protocol for using the QuantiFluor™ dsDNA System with the QuantiFluor™-ST Fluorometer and the PCR Tube Adapter. The QuantiFluor™ PCR Tube Adapter allows for sample volumes as little as 100µl without sacrificing instrument sensitivity.

Related Products