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QuantiFluor™-ST Fluorometer Method for 4-Methylumbelliferone

Abstract

The QuantiFluor™-ST Fluorometer provides sensitive, reliable measurements of 4-methylumbelliferone (4-MU) released during β-galactosidase (GAL) cleavage. Using standard 10x10mm cuvettes, the QuantiFluor™-ST instrument has a linear detection range from 750 nanomolar down to 0.1 nanomolar, or 20fg/ml 4-MU. Sensitivity levels for the minicell adaptor range from 200nM down to 1.0nM.