Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

QuantiFluor™-ST Fluorometer Method For DNA Quantitation Using Hoechst 33258

Abstract

As with the common practice of visualizing DNA in a gel with ethidium bromide, quantitation of DNA can be easily achieved in a fluorometer with the dye, Hoechst 33258, a bisbenzimide DNA intercalator that excites in the near UV (350nm) and emits in the blue region (450nm). Sensitivity of the Hoechst 33258 assay is approximately 10ng/ml when it is used in conjunction with the  QuantiFluor™-ST Fluorometer.