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QuantiFluor® dsDNA System Technical Manual

Instructions for Use of Product(s)

Literature # TM346

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye (504nmEx/531nmEm) that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). This dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.

The QuantiFluor® dsDNA Dye was developed using the fluorescence module of GloMax® Multi+ Detection System and the QuantiFluor®-ST Handheld Fluorometer. The Quantus™ Fluorometer was optimized for use with the QuantiFluor® Dyes, including the QuantiFluor® dsDNA Dye. The QuantiFluor® dsDNA System can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths.

Summary of Changes
The following changes were made to the 12/18 revision of this document:
1. Updated cross-references and section numbering.

Printed in USA. Revised 12/18.

Experienced User Protocols