CD28 Blockade Bioassay Technical Manual
Instructions for Use of Product(s)
JA6101, JA6105, JA7101, JA7105
Literature # TM606
The CD28 Blockade Bioassay is a bioluminescent reporter cell-based assay that can be used to measure the potency and stability of antibodies and other potential biologics that block CD28/B7. The assay consists of two genetically engineered cell lines, CD28 Effector Cells and aAPC/Raji Cells. These cells are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation.
When the two cell types are co-cultured, the aAPC/Raji Cells activate TCR/CD3 and CD28 on the Effector Cells to induce maximum promoter-mediated luminescence. Addition of a biologic that blocks CD28/B7 inhibits co-stimulation by CD28 and results in decreased promoter-mediated luminescence. The bioluminescent signal is quantified using the Bio-Glo™ Luciferase Assay System, and a standard luminometer such as the GloMax® Discover System.
This Technical Manual is relevant for Cat.# JA6101 and JA6105; JA7101 and JA7105 (Korea).
Summary of Changes
The following changes were made to the 4/25 revision of this document:
1. Removed an expired patent statement.
2. Revised text about the label in Section 3.
Revised 4/25.