NanoBRET® TE K192 Kinase Selectivity System Technical Manual
Instructions for Use of Product(s)
NP4100, NP4101, NP4050, NP4060
Literature # TM683
The NanoBRET® TE K192 Kinase Selectivity System provides a novel microplate-based workflow that determines live-cell compound occupancy across 192 kinases in a single experiment, using common laboratory equipment. The system includes the NanoBRET® TE K192 Kinase Vector Panel and the NanoBRET® TE Intracellular Kinase Assay K-10. The NanoBRET® TE K192 Kinase Vector Panel contains 192 unique kinase-NanoLuc® luciferase fusion vectors that are arrayed in 96-well plates and are transfection ready. On day 1 of the experiment, the user transfects the K192 vector panel into HEK293 cells. Each kinase expressed by a vector within this panel is full-length. The NanoBRET® TE Intracellular Kinase Assay, K-10, contains the necessary reagents to run the 192 kinase NanoBRET® TE assays in a single experiment on day 2. NanoBRET® Tracer K-10 has been optimized for maximum kinome coverage, enabling a single-reagent design for broad spectrum profiling. Furthermore, each kinase within the NanoBRET® Target Engagement K192 Kinase Vector Panel uses the NanoBRET® Tracer K-10 at one of four concentrations. The tracer concentrations were optimized to provide quantitative occupancy data for each kinase, further simplifying the assay.
Summary of Changes
The following changes were made to the 9/25 revision of this document:
1. Removed two expired patent statements and updated other patent statements.
2. Updated the NanoBRET and Vivazine trademarks to registered.
3. Updated the Limited Use Label Licenses to reflect a change to NanoBRET trademark.
4. Updated a third-party trademark.
Revised 9/25.