GITR Bioassay Technical Manual
Instructions for Use of Product(s)
JA2291, JA2295, JA3291, JA3295, JA2251, JA2255
Literature # TM582
The GITR Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate GITR. The assay consists of a genetically engineered Jurkat T cell line that expresses human GITR and a luciferase reporter driven by a response element that can respond to GITR ligand/agonist antibody stimulation. The GITR Effector Cells are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation.
This Technical Manual is relevant for Cat.# JA2291 and JA2295; JA3291 and JA3295 (Korea).
Summary of Changes
The following changes were made to the 6/25 revision of this document:
1. Revised text about the label in Section 3.
2. Removed an expired patent statement and revised another patent.
3. Updated the fonts and the cover image.
Revised 6/25.