We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

FcγRIIb CHO-K1 Cells

Measure potency and stability of agonist antibodies that are FcγRIIb crosslinking-dependent

  • Used with co-stimulatory bioassays to crosslink antibodies that stimulate T cell activation
  • Thaw-and-use format to achieve consistency
  • Mechanism-of-action-based assay

Choose an option

Catalog number selected: JA2251

$ 475.00
Your price:
Add to Cart
This product is discontinued
FcγRIIb CHO-K1 Cells
1 each
$ 475.00
Your price: Log in
Change Configuration

Measure FcγRIIb Crosslinking Dependence of Agonist Antibodies

The antibodies used to stimulate T cell activation via interaction with co-stimulatory receptors on the effector cells can be cross-linked via binding to FcγRIIb receptors on CHO-K1 cells. Based on the properties of the antibody tested, the Co-Stimulatory Immune Checkpoint (i.e., OX40, CD40, GITR and 4-1BB) bioassays can be conducted in a single genetically engineered cell system or dual genetically engineered cell systems.

FcγRIIb CHO-K1 Cells are engineered to express human FcγRIIb, provided in cryopreserved thaw-and-use format. These are not reporter cells, and function solely to crosslink anti-CD40, anti-OX40, anti-4-1BB or anti-GITR antibodies.

The Workflow

Traditional Method

Standard bioassay workflow with cultured cells

Standardized Reagents Improve Results

  • Thaw-and-use cell format provides significant time and labor savings over traditional methods while reducing variability.
  • Kit includes all of the required reagents in standardized formats.
  • Easily amenable to high-throughput workflows.

'Cells as Reagents' Method

Improved bioassay workflow with thaw-and-use cells

The Assay Principle

15384ma

Representation of co-stimulatory bioassays (CD40, OX40, GITR or 4-1BB) with FcγRIIb-independent and FcγRIIb-dependent antibodies.  Panel A. Assay with FcγRIIb-independent Antibody. The bioassay consists of one engineered cell line: CD40, OX40, GITR or 4-1BB Effector Cells. In the absence of agonist antibody or ligand, the CD40, OX40, GITR or 4-1BB receptor is not activated and luminescence signal is low. The addition of agonist antibody or ligand induces the CD40, OX40, GITR or 4-1BB pathway-activated luminescence, which can be detected in a dose-dependent manner by addition of Bio-Glo™ Reagent and quantitation with a luminometer. Panel B. Assay with FcγRIIb-dependent Antibody. The bioassay consists of two engineered cell lines: CD40, OX40, GITR or 4-1BB Effector Cells and FcγRIIb CHO-K1 Cells. In the presence of FcγRIIb CHO-K1 Cells, the anti-CD40, -OX40, -GITR or 4-1BB antibody is cross-linked, thereby inducing CD40, OX40, GITR or 4-1BB pathwayactivated luminescence, which can be detected in a dose-dependent manner by addition of Bio-Glo™ Reagent and quantitation with a luminometer.

  • Kits are available in 1X and 5X sizes.
  • 4-1BB, CD40, OX40 and GITR Bioassays are available separately.

Specifications

You are viewing: JA2251 Change Configuration

What's in the box?

Item Part # Size

FcγRIIb CHO-K1 Cells

J225A 1 × 0.5ml

RPMI 1640 Medium

G708A 1 × 36ml

SDS

Choose language:

Download SDSPDF (288 KB) – English (United States)

Certificate of Analysis

See all certificates for JA2251

Use Restrictions

Not For Medical Diagnostic Use.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: JA2255 Change Configuration

What's in the box?

Item Part # Size

FcγRIIb CHO-K1 Cells

J225A 5 × 0.5ml

RPMI 1640 Medium

G708A 5 × 36ml

SDS

Choose language:

Download SDSPDF (289 KB) – English (United States)

Certificate of Analysis

See all certificates for JA2255

Use Restrictions

Not For Medical Diagnostic Use.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Let's find the product that meets your needs.

Talk to a Scientist

Sabine

Sabine

Germany