mFcγRIV ADCC Reporter Bioassay, Complete Kit, Technical Manual
Instructions for Use of Product(s)
M1201, M1301, M1401
Literature # TM485
The mFcγRIV ADCC Reporter Bioassay, Complete Kit, is a bioluminescent cell-based assay that overcomes the limitations of existing antibody-dependent cell-mediated cytotoxicity (ADCC) assays and can be used to measure the potency and stability of antibodies and other biologics with Fc domains that specifically bind and activate murine FcγRIV (mFcγRIV). mFcγRIV is the predominant receptor involved in ADCC in the mouse and is more closely related to human FcγRIIIa, the primary Fc receptor involved in ADCC in humans, than mFcγRIII. The assay consists of a genetically engineered Jurkat T cell line that expresses:
- Mouse FcγRIV receptor
- A luciferase reporter driven by an NFAT-response element (NFAT-RE)
The mFcγRIV Effector Cells are provided in thaw-and-use format, which includes cryopreserved cells that can be thawed, plated and used in an assay without the need for propagation.
This Technical Manual is relevant for Cat.# M1201; M1301 (Taiwan); and M1401 (Korea).
Printed in USA 12/16.