Magne™ Protein A Beads and Magne™ Protein G Beads for Antibody Purification Technical Manual
Literature # TM371
Magne™ Protein A Beads and Magne™ Protein G Beads are magnetic affinity beads with high specificity and high capacity for binding immunoglobulins from cell culture, ascites and serum samples. The beads have excellent magnetic properties that allow rapid and efficient capture using a variety of magnetic stands. Antibody purification can be performed using a single sample or multiple samples in parallel, with sample volumes of 20µl to 50ml.
Magne™ Protein A Beads and Protein G Beads beads use a novel attachment chemistry based on the HaloTag® technology to immobilize Protein A or Protein G. The HaloTag® protein is a multi-functional protein tag that forms a covalent bond with its ligand and is used for protein expression and purification, cell imaging, protein immobilization and protein-interaction analysis. Magne™ Protein A Beads and Magne™ Protein G Beads use recombinant Protein A from Staphylococcus and Protein G from Streptococci, respectively, fused with the HaloTag® protein. The Protein A-HaloTag® (M.Wt 71kDa) or Protein G-HaloTag® (M.Wt 58kDa) fusion protein is covalently attached in an oriented fashion to a magnetic cellulose bead that is activated using a HaloTag® ligand.
The Magne™ Protein A and Protein G Beads are not recommended for use in immunoprecipitation (IP) or co-IP applications.
Summary of Changes
At the 7/15 revision, the document design was updated.
Printed in USA. Revised 7/15.