CellTox™ Green Cytotoxicity Assay Technical Manual

Instructions for Use of Product(s)
G8731, G8741, G8742, G8743
Literature # TM375

The CellTox™ Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended for assessing cytotoxicity in cell culture after experimental manipulation. The assay system uses a proprietary asymmetric cyanine dye that is excluded from viable cells but preferentially stains the dead cell DNA. When the dye binds DNA in compromised cells, its fluorescence properties are substantially enhanced. Viable cells produce no appreciable increases in fluorescence. Therefore, the fluorescence signal produced by the binding interaction with dead cell DNA is proportional to cytotoxicity.

Summary of Change
The following change was made to the 5/15 revision of this document:
The Notes referring to the optional toxicity control in Sections 5.A–D were revised.

Revised 5/15.