We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

ADCC Reporter Bioassay Core Kit Technical Manual

Instructions for Use of Product(s)
G7010, G7018
Literature # TM383

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action of antibodies through which virus-infected or other diseased cells are targeted for destruction by components of the cell-mediated immune system, such as natural killer cells. The ADCC Reporter Bioassay is a bioluminescent reporter assay for quantifying biological activity on pathway activation by therapeutic antibody drugs in an ADCC mechanism of action (MOA) assay. The assay combines a simple, add-mix-read format, effector cells provided in frozen, thaw-and-use format, and an optimized protocol to provide a bioassay that has low variability and high accuracy. Moreover the bioassay can be performed in a single day. These performance characteristics make the bioassay suitable for applications across antibody drug research, development, and manufactured lot release. The thaw-and-use cells provided in the ADCC Reporter Bioassay kits are generated under highly controlled conditions that drive low assay variability run to run, while providing the convenience of an assay reagent that eliminates the need to propagate and prepare cells each time.

Summary of Changes
The following changes were made to the 9/18 revision of this document:
1. Removed reference to discontinued instrument.

2. Added product to Related Products.

Printed in USA. Revised 9/18.

Download video:

Closed format: MP4
Open format: OGG