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ADCC Bioassay Effector Cells, Propagation Model

Instructions for Use of Product(s)

Literature # TM385

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action of antibodies through which virus-infected or other diseased cells are targeted for destruction by components of the cell-mediated immune system, such as natural killer cells. The ADCC Reporter Bioassay is a bioluminescent reporter assay for quantifying biological activity on pathway activation by therapeutic antibody drugs in an ADCC mechanism of action (MOA) assay. The ADCC Reporter Bioassay can be performed with the ADCC Bioassay Effector Cells, Propagation Model, described here, a model that allows cell banking and propagation under a unique purchase agreement. The assay combines a simple, add-mix-read format and an optimized protocol to provide a bioassay that has low variability and high accuracy. These performance characteristics make the bioassay suitable for application across antibody drug research, development and manufactured lot release.

Summary of Changes
The following change was made to the 3/19 revision of this document:
1. Corrected the HyClone fetal bovine serum catalog number in Section 3.A.

Revised 3/19