Kinase-Glo® Luminescent Kinase Assay Platform Technical Bulletin
Literature # TB372
The Kinase-Glo® Luminescent Kinase Assay Platform provides a homogeneous, high-throughput screening method for measuring kinase activity by quantitating the amount of ATP remaining in solution following a kinase reaction. The Kinase-Glo® Platform consists of three assay formats: the Kinase-Glo® Assay, which is used to monitor kinase activity using up to 10µM ATP; the Kinase-Glo® Plus Assay, which is used for assays requiring higher ATP concentrations (up to 100µM); and the Kinase-Glo® Max Assay, which is used for assays requiring up to 500µM ATP. The Kinase-Glo® Assays are performed in a single well of a multiwell plate by adding a volume of Kinase-Glo® Reagent equal to the volume of a completed kinase reaction and measuring luminescence. The luminescent signal is correlated with the amount of ATP present and is inversely correlated with the amount of kinase activity.
Summary of Changes
The 2/15 version of this technical manual was modified to update patent and disclaimer statements.
Printed in USA. Revised 2/15.