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CytoTox-Glo™ Cytotoxicity Assay Technical Bulletin

Instructions for Use of Product(s)
G9290, G9291, G9292

Literature # TB359

The CytoTox-Glo™ Cytotoxicity Assay uses a luminogenic peptide substrate, the AAF-Glo™ Substrate, to measure dead-cell protease activity, which is released from cells that have lost membrane integrity. The AAF-Glo™ Substrate cannot cross the intact membrane of live cells and does not generate any appreciable signal from the live-cell population. The CytoTox-Glo™ Assay relies on the properties of Ultra-Glo™ Recombinant Luciferase, which uses aminoluciferin as a substrate to generate a stable "glow-type" luminescent signal and is formulated to improve performance across a wide range of assay conditions.

Summary of Changes
The following change was made to the 9/17 revision of this document:
The viability equation on p. 10 was revised.

Printed in USA. Revised 9/17.