cAMP-Glo™ Assay Technical Bulletin
Literature # TB357
The cAMP-Glo™ Assay is a homogeneous, bioluminescent and high-throughput assay to measure cAMP levels in cells. The cAMP-Glo™ Assay monitors cAMP production in cells in response to the effects of an agonist or test compound on G protein-coupled receptors (GPCR). GPCRs that couple with adenylate cyclase will increase or decrease intracellular cAMP. The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.
Summary of Changes
The following change was made to the 6/17 revision of this document:
Corrected the treatment for suspension cells in Section 3.
Printed in USA. Revised 6/17.