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cAMP-Glo™ Assay

Simple, Bioluminescent cAMP Detection

  • Complete in ~45 minutes
  • Scalable to 1536-well plate formats and beyond
  • No interference by fluorescent compounds

Size

Catalog number selected: V1501

$ 375.00
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cAMP-Glo™ Assay
300 assays
$ 375.00
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Monitor Effect of Test Compounds on GPCRs

The cAMP-Glo™ Assay is designed to monitor cAMP production in response to the effects of test compounds on G protein-coupled receptors (GPCR). GPCRs that couple with adenylate cyclase will increase or decrease intracellular cAMP. The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.

The cAMP-Glo™ Assay can be performed in 96-, 384- or 1536-well plates. The cells are induced with a test compound for an appropriate period of time to modulate cAMP levels. After induction, cells are lysed to release cAMP, then the cAMP detection solution, which contains protein kinase A, is added. Kinase-Glo® Reagent is then added to terminate the PKA reaction and detect the remaining ATP via a luciferase reaction. Plates are read using a microplate-reading luminometer. Luminescence can be correlated to cAMP concentrations using a cAMP standard curve. The half-life for the luminescent signal is greater than 4 hours. This extended signal half-life eliminates the need for luminometers with reagent injectors and allows batch-mode processing of multiple plates. 

Cat.# V1501 contains sufficient reagents to perform 300 assays at 60μl final volume per well in 384-well plates.
Cat.# V1502 contains sufficient reagents to perform 3,000 assays at 60μl final volume per well in 384-well plates.
Cat.# V1503 contains sufficient reagents to perform 30,000 assays at 60μl final volume per well in 384-well plates.

Excellent Signal:Background

The assay provides the best signal:background ratio of all the cAMP assays; signal:background >200 (with cAMP), >15 (on cells).

Proven Luminescence Technology

Powered by Ultra-Glo™ Recombinant Luciferase, the bioluminescence-based cAMP-Glo™ assay provides a safe alternative to radioactive methods, and avoids the interference issues that can be problematic for fluorescence-based methods.

Representation of cAMP production in cells and the cAMP-Glo™ Assay.

Representation of cAMP production in cells and the cAMP-Glo™ Assay.
Representation of cAMP production in cells and the cAMP-Glo™ Assay.

Specifications

You are viewing: V1501 Change Configuration

What's in the box?

Item Part # Size

Protein Kinase A

V135D 1 × 16μl

cAMP-Glo™ Lysis Buffer

V151A 1 × 5ml

cAMP-Glo™ Reaction Buffer

V152A 1 × 8ml

cAMP, 1mM

V642B 1 × 100μl

Kinase-Glo® Substrate

V672A 1 × 1 vial

Kinase-Glo® Buffer

V673A 1 × 10ml

SDS

Choose language:

Download SDSPDF (221 KB) – English (United States)

Certificate of Analysis

See all certificates for V1501

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Patent Pending.

Specifications

You are viewing: V1502 Change Configuration

What's in the box?

Item Part # Size

Protein Kinase A

V135C 1 × 160μl

cAMP-Glo™ Lysis Buffer

V151B 1 × 25ml

cAMP-Glo™ Reaction Buffer

V152B 1 × 50ml

cAMP, 1mM

V642B 1 × 100μl

Kinase-Glo® Substrate

V672B 1 × 1 vial

Kinase-Glo® Buffer

V673B 1 × 100ml

SDS

Choose language:

Download SDSPDF (226 KB) – English (United States)

Certificate of Analysis

See all certificates for V1502

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Patent Pending.

Specifications

You are viewing: V1503 Change Configuration

What's in the box?

Item Part # Size

Protein Kinase A

V135C 10 × 160μl

cAMP-Glo™ Lysis Buffer

V151C 1 × 250ml

cAMP-Glo™ Reaction Buffer

V152C 1 × 500ml

cAMP, 1mM

V642A 1 × 500μl

Kinase-Glo® Substrate

V672B 10 × 1 vial

Kinase-Glo® Buffer

V673B 10 × 100ml

SDS

Choose language:

Download SDSPDF (226 KB) – English (United States)

Certificate of Analysis

See all certificates for V1503

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Patent Pending.

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