CytoTox 96® Non-Radioactive Cytotoxicity Assay Technical Bulletin
Instructions for Use of Product(s)
G1780
Literature # TB163
The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to 51Cr release cytotoxicity assays. The assay measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected using a standard 96-well plate reader.
Summary of Changes
The following changes were made to the 8/25 revision of this document:
1. Updated fonts and the cover image.
2. Made minor text edits including updating storage temperature ranges per our current format.
3. Applications list was removed, including related references. Remaining references were renumbered.
4. Updated Related Products, Section 10.B.
Revised 8/25.