We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Use code TVECTOR20 at checkout to get 20% off pGEM®-T Vector Systems! Shop Now ›

CytoTox 96® Non-Radioactive Cytotoxicity Assay

A Colorimetric Alternative to Radioactive Cytotoxicity Assays

  • Measures release of LDH, an indicator of cytotoxicity
  • Non-radioactive, time-saving assay


Catalog number selected: G1780

$ 340.00
Your price:
Add to Cart
This product is discontinued
CytoTox 96® Non-Radioactive Cytotoxicity Assay
1,000 assays
$ 340.00
Your price: Log in

The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected using a standard 96-well plate reader. The assay can be used to measure membrane integrity for cell-mediated cytotoxicity assays in which a target cell is lysed by an effector cell, or to measure lysis of target cells by bacteria, viruses, proteins, chemicals, etc.

The CytoTox 96® Non-Radioactive Cytotoxicity Assay requires no radioactive waste disposal or [51Cr] and eliminates labeling of target cells prior to conducting experiments. Standard equipment can be used to collect absorbance (visible wavelength) data with a standard 96-well plate reader. The assay can be used for a variety of applications including measurement of 1) cell-mediated cytotoxicity; 2) chemical-mediated cytotoxicity; and 3) total cell number. A highly sensitive assay, it can reveal early, low-level damage to cell membranes that is often missed with other methodologies.


What's in the box?

Item Part # Size

Substrate Mix

G179A 5 × 1 vial

Assay Buffer

G180A 1 × 60ml

LDH Positive Control

G181A 1 × 25μl

Lysis Solution, 10X

G182A 1 × 5ml

Stop Solution

G183A 1 × 65ml


Choose language:

Download SDSPDF (147 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Store LDH Positive Control, Lysis Solution (10X) and Stop Solution at 4°C.


Let's find the product that meets your needs.

Talk to a Scientist