CellTiter 96® AQueous One Solution Cell Proliferation Assay System Technical Bulletin

Instructions for Use of Product(s)
G3580, G3581, G3582

Literature # TB245

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method to determine the number of viable cells in proliferation or cytotoxicity assays. The CellTiter 96® AQueous One Solution Reagent contains a novel tetrazolium compound [3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine ethosulfate; PES). PES has enhanced chemical stability, which allows it to be combined with MTS to form a stable solution. This convenient "One Solution" format is an improvement over the first version of the CellTiter 96® AQueous Assay, where phenazine methosulfate (PMS) is used as the electron coupling reagent, and PMS Solution and MTS Solution are supplied separately.

Summary of Changes
The following changes were made to the 11/23 revision of this document:
1. Updated Section 6.
2. Updated patent statements.
3. Changed font and cover image.
4. Made minor text edits.

Revised 11/23.