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T4 DNA Ligase, Blue/White Cloning Qualified Protocol

Instructions for Use of Product(s)
M1801, M1804, M1794

Literature # 9PIM180

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule, but will not join single-stranded nucleic acids.

Revised 4/18.