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GoTaq® Hot Start Polymerase Product Information

Instructions for Use of Product(s)
M5001, M5005, M5006, M5008

Literature # 9PIM500

GoTaq® Hot Start Polymerase contains high-performance GoTaq® DNA polymerase bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, for convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields.

Revised 4/18.