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GoTaq® Hot Start Polymerase

M5001_GoTaq--Hot-Start-Polymerase--100u_3
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Robust Hot Start PCR with Convenient Room-Temperature Setup

  • Hot Start Taq minimizes nonspecific amplification and primer-dimers
  • Optimize reaction conditions with supplied Flexi Reaction Buffer and MgCl2 solution
  • Direct-to-gel convenience
  • Supplied at a concentration of 5u/µl

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Catalog number selected: M5001

$ 91.00
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GoTaq® Hot Start Polymerase
100u
$ 91.00
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High-Performance Hot-Start PCR 

GoTaq® Hot Start Polymerase contains high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase also exhibits 5´→3´ exonuclease activity. The enzyme is supplied with a tube of 25mM MgCl2 to optimize the magnesium concentration in your reactions.

GoTaq® Hot Start Polymerase is supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.

pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

GoTaq Hot Start Polymerase with Colorless Flexi Buffer (C) and with Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.

GoTaq® Hot Start Polymerase with Colorless Flexi Buffer (C) and Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.

Specifications

You are viewing: M5001 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

MgCl2

A351B 1 × 750μl 25mM View Product

GoTaq® Hot Start Polymerase

M500A 1 × 100u

5X Colorless GoTaq® Flexi Buffer

M890A 1 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 1 × 1ml View Product

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: M5005 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

MgCl2

A351H 3 × 1.2ml 25mM View Product

GoTaq® Hot Start Polymerase

M500B 1 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 4 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 4 × 1ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: M5006 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

MgCl2

A351H 15 × 1.2ml 25mM View Product

GoTaq® Hot Start Polymerase

M500B 5 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 20 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 20 × 1ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: M5008 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

MgCl2

A351H 60 × 1.2ml 25mM View Product

GoTaq® Hot Start Polymerase

M500B 20 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 80 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 80 × 1ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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