Product Availability Update

Promega Manufacturing and Delivery Systems continue to be fully operational during the COVID-19 outbreak. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Learn more

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

GoTaq® Flexi DNA Polymerase (M829) Protocol

Instructions for Use of Product(s)
M8291, M8295, M8296, M8297, M8298

Literature # 9PIM829

GoTaq® Flexi DNA Polymerase offers robust amplification equal to and in some cases superior to conventional Taq DNA polymerase, allows optimization of MgCl2 concentration, and facilitates direct-to-gel analysis of PCR products. The enzyme is supplied with a tube of 25mM MgCl2, allowing you to optimize the magnesium concentration in your reactions. Two reaction buffers, Green and Colorless GoTaq® Flexi Buffers, are also supplied. Both buffers contain a compound that increases sample density, allowing samples to sink easily into the wells of agarose gels and making it possible to load your reaction directly onto a gel after amplification. The 5X Green GoTaq® Flexi Buffer contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA.

Revised 4/18.