GoTaq® Flexi DNA Polymerase (M829) Protocol
Literature # 9PIM829
GoTaq® Flexi DNA Polymerase offers robust amplification equal to and in some cases superior to conventional Taq DNA polymerase, allows optimization of MgCl2 concentration, and facilitates direct-to-gel analysis of PCR products. The enzyme is supplied with a tube of 25mM MgCl2, allowing you to optimize the magnesium concentration in your reactions. Two reaction buffers, Green and Colorless GoTaq® Flexi Buffers, are also supplied. Both buffers contain a compound that increases sample density, allowing samples to sink easily into the wells of agarose gels and making it possible to load your reaction directly onto a gel after amplification. The 5X Green GoTaq® Flexi Buffer contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA.