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Cell-Based Proteasome-Glo™ Assays

A0003

Homogeneous, Luminescent Assays that Measure Protease Activities in Cultured Cells

  • Simple "add-mix-measure" protocol
  • Chymotrypsin-Like, Trypsin-Like and Caspase-Like activity data directly from cellular environment
  • Results within 10-30 minutes after reagent addition

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Catalog number selected: G8660

$ 500.00 Your price: Log In

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Cell-Based Proteasome-Glo™ Assays
Chymotrypsin-like/10ml
$ 500.00
Your price: Log In
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Measure Protease Activity in Cultured Cells

Homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells for more biologically relevant results. These assays can also be used to screen inhibitor libraries and monitor proteasome-regulated protein degradation in cells. Proteasome-Glo™ Cell-Based Assays provide luminogenic proteasome substrates in buffers optimized for cell permeabilization, proteasome activity and luciferase activity. Addition of the Proteasome-Glo™ Cell-Based Reagent in an "add-mix-measure" format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.

The three luminogenic substrates used to monitor specific protease activities include: Suc-LLVY-aminoluciferin for chymotrypsin-like, Z-LRR-aminoluciferin for trypsin-like, and Z-nLPnLD-aminoluciferin for caspase-like activity. Each luminogenic substrate is added to a buffer system optimized for its specific proteasome activity and luciferase activity. The reagents are added to cells in culture, and the proteasome cleaves the substrates, releasing luciferin, which is consumed by luciferase, producing "glow-type" luminescence correlating to enzyme activity or inhibition. The 26S proteasome is a 2.5MDa multiprotein complex found in all eukaryotic cells.

The Proteasome-Glo™ Cell-Based 3-Substrate System consists of three homogeneous bioluminescent assays that measure the three proteolytic activities associated with the proteasome in a cell-based format (each of these three assays also is available separately). The simplified method minimizes handling steps and makes the assays amenable to automation. The luminescent assay format avoids inherent fluorescent background signals, providing excellent signal-to-background readings. The assays are miniaturizable to 384-well format.  

5855MA-W
The luminogenic substrates containing the Suc-LLVY, Z-LRR or Z-nLPnLD sequence are recognized by the 20S proteasome.
Luminescent signal is proportional to cell number and linear over three logs of cell number (r2 = 0.99, slope = 0.94).
5732MA-W
Monitoring proteasome inhibition using different inhibitors and cell lines.
5734MA-W-a
5734MA-W-b
5734MA-W-c

Number of Assays Per Plate

Product Cat.# # Assays Volume/Assay Plate Size
Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay
G8660 100 100µl 96-well
400 25µl 384-well
Proteasome-Glo™ Trypsin-Like Cell-Based Assay
G8760 100 100µl 96-well
400 25µl 384-well
Proteasome-Glo™ Caspase-Like Cell-Based Assay
G8860 100 100µl 96-well
400 25µl 384-well
Proteasome-Glo™ 3-Substrate Cell-Based Assay System
G1180 100* 100µl 96-well
400* 25µl 384-well
G1200 500* 100µl 96-well
2,000* 25µl 384-well

*For each of the 3 systems.

Protocols

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Suc-LLVY-Glo™ Substrate

G856C 1 x 50μl

Proteasome-Glo™ Cell-Based Buffer

G865A 1 x 10ml

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 5 × 10ml Change Configuration

What's in the box?

Item Part # Size

Suc-LLVY-Glo™ Substrate

G856C 5 x 50μl

Proteasome-Glo™ Cell-Based Buffer

G865A 5 x 10ml

Luciferin Detection Reagent

V859A 5 x 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 2 × 50ml Change Configuration

What's in the box?

Item Part # Size

Suc-LLVY-Glo™ Substrate

G856D 2 x 250μl

Proteasome-Glo™ Cell-Based Buffer

G865B 2 x 50ml

Luciferin Detection Reagent

V859B 2 x 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Inhibitor 1

G166A 1 x 15μl

Inhibitor Mix 2

G167A 1 x 100μl

Z-LRR-Glo™ Substrate

G855A 1 x 100μl

Proteasome-Glo™ Cell-Based Buffer

G865A 1 x 10ml

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 5 × 10ml Change Configuration

What's in the box?

Item Part # Size

Inhibitor 1

G166A 5 x 15μl

Inhibitor Mix 2

G167A 5 x 100μl

Z-LRR-Glo™ Substrate

G855A 5 x 100μl

Proteasome-Glo™ Cell-Based Buffer

G865A 5 x 10ml

Luciferin Detection Reagent

V859A 5 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Z-nLPnLD-Glo™ Substrate

G857A 1 x 50μl

Proteasome-Glo™ Cell-Based Buffer

G865A 1 x 10ml

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 5 × 10ml Change Configuration

What's in the box?

Item Part # Size

Z-nLPnLD-Glo™ Substrate

G857A 5 x 50μl

Proteasome-Glo™ Cell-Based Buffer

G865A 5 x 10ml

Luciferin Detection Reagent

V859A 5 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

G8660 1 x 10ml

Proteasome-Glo™ Trypsin-Like Cell-Based Assay

G8760 1 x 10ml

Proteasome-Glo™ Caspase-Like Cell-Based Assay

G8860 1 x 10ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: 50ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

G8661 1 x 5 × 10ml

Proteasome-Glo™ Trypsin-Like Cell-Based Assay

G8761 1 x 5 × 10ml

Proteasome-Glo™ Caspase-Like Cell-Based Assay

G8861 1 x 5 × 10ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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