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Calpain-Glo™ Protease Assay

Homogeneous, Luminescent Assay that Measures Calpain 1 (μ) and 2 (m) Activities

  • Simple "add-mix-measure" protocol
  • Maximum sensitivity while enzyme is still active
  • 1,000x more sensitive than fluorometric assays

Size

Catalog number selected: G8501

$ 360.00
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Calpain-Glo™ Protease Assay
10ml
$ 360.00
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Simple Protocol Offers Fast, Sensitive Results

The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities. Calpains are a family of calcium-activated cysteine proteases involved in cleaving a wide variety of proteins. Calpains modulate the biological activities of their substrates via limited proteolysis.

The Calpain-Glo™ Protease Assay provides a succinyl, proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimized for calpain and luciferase activities. The addition of the calpain reagent in an "add-mix-measure" format results in calpain cleavage of the substrate and rapid development of a "glow-type" luminescent signal produced by the luciferase reaction. The signal is proportional to the amount of calpain activity present. The assay is designed for use with purified enzyme preparations.

  • Designed for measuring calpain activity and/or inhibition in a multiwell format and easy to automate.
  • Well suited for rapidly autolysed enzymes like calpain; maximum sensitivity is reached in as little as 10 minutes, while the enzyme is fully active.
  • Up to 1,000 times more sensitive than competitive fluorometric assays. The luminescent assay avoids inherent fluorescent background signals, providing excellent signal-to-background readings. The assay is linear over 4 logs of calpain concentration.
Reference
  1. Moldoveanu, T. et al. (2002) Cell 108, 649–60.
5108MA-W
Sensitivity of the Calpain-Glo™ Protease Assay compared to fluorescent assays.

Specifications

You are viewing: G8501 Change Configuration

What's in the box?

Item Part # Size

Calpain-Glo™ Buffer

G852A 1 × 10ml

Suc-LLVY-Glo™ Substrate

G856A 1 × 100μl

Luciferin Detection Reagent

V859A 1 × 1 each

SDS

Choose language:

Download SDSPDF (93 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: G8502 Change Configuration

What's in the box?

Item Part # Size

Calpain-Glo™ Buffer

G852B 1 × 50ml

Suc-LLVY-Glo™ Substrate

G856B 1 × 500μl

Luciferin Detection Reagent

V859B 1 × 1 each

SDS

Choose language:

Download SDSPDF (90 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Resources

Citations

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