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Riboprobe® Systems

Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts

  • Systems available with SP6 RNA Polymerase, T7 RNA Polymerase or T3 RNA Polymerase
  • SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
  • Allow synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct

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Catalog number selected: P1420

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Riboprobe® Systems
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$ 172.00
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The Riboprobe® Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. These systems contain all components necessary for in vitro transcription from a DNA template (excluding the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for template removal following transcription.

Applications of the Riboprobe® Systems include:

  • Generation of high-specific-activity RNA probes.
  • Generation of microgram quantities of RNA transcripts.
  • Generation of RNA processing substrates.
  • Generation of antisense RNA.

Protocols

Specifications

You are viewing: 1 system Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

RQ1 RNase-Free DNase

M610C 1 x 110u View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 x 2,500u

SP6 RNA Polymerase

P108E 1 x 500u

rATP

P113A 1 x 50μl10mM View Product

rCTP

P114A 1 x 50μl10mM View Product

rGTP

P115A 1 x 50μl10mM View Product

rUTP

P116A 1 x 50μl10mM View Product

DTT

P117A 1 x 100μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 x 500μl View Product

Nuclease-Free Water

P119A 1 x 1,250μl View Product

pGEM® Express Positive Control Template

P256A 1 x 5μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Specifications

You are viewing: 1 system Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

RQ1 RNase-Free DNase

M610C 1 x 110u View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 x 2,500u

rCTP

P114A 1 x 50μl10mM View Product

rATP

P113A 1 x 50μl10mM View Product

rGTP

P115A 1 x 50μl10mM View Product

rUTP

P116A 1 x 50μl10mM View Product

DTT

P117A 1 x 100μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 x 500μl View Product

Nuclease-Free Water

P119A 1 x 1,250μl View Product

T3 RNA Polymerase

P208A 1 x 500u

pGEM® Express Positive Control Template

P256A 1 x 5μg

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Specifications

You are viewing: 1 system Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

RQ1 RNase-Free DNase

M610C 1 x 110u View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 x 2,500u

rATP

P113A 1 x 50μl10mM View Product

rCTP

P114A 1 x 50μl10mM View Product

rGTP

P115A 1 x 50μl10mM View Product

rUTP

P116A 1 x 50μl10mM View Product

DTT

P117A 1 x 100μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 x 500μl View Product

Nuclease-Free Water

P119A 1 x 1,250μl View Product

T7 RNA Polymerase

P207D 1 x 500u

pGEM® Express Positive Control Template

P256A 1 x 5μg

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

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