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T7 RNA Polymerase

DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences

  • >90% pure as determined by SDS polyacrylamide gel electrophoresis
  • Free of detectable levels of contaminating RNase and DNase activity (<1% release)
  • Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates
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Catalog number selected: P2075

$ 78.00 Your price: Log in

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T7 RNA Polymerase
10–20u/µl/1,000u
$ 78.00
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T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis. T7 RNA Polymerase is provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM spermidine, 50mM NaCl. Cat.# P4074 provides T7 RNA Polymerase at a high concentration (80u/µl).

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Applications

  • Generation of templates for in vitro translation.
  • Generation of probes for nucleic acid hybridizations.
  • Generation of RNA processing substrates.
  • Generation of antisense RNA.

References

  1. Milligan, J.F. et al. (1987) Nucl. Acids. Res. 15, 8783–98.
  2. Milligan, J.F. and Uhlenbeck. O.C. (1989) Meth. Enzymol. 180, 51–62.

Storage Buffer: 20mM potassium phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCI, 0.1% (v/v) Triton® X-100 and 50% (v/v) glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100μl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2,10mM DTT, 10mM NaCl2, 2mM spermidine, 0.05% Tween® 20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5μCi [3H]CTP and 2μg supercoiled pGEM®-5Zf(+) Vector DNA.

Protocols

Specifications

You are viewing: P2075 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

DTT

P117B 1 × 250μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 × 500μl View Product

T7 RNA Polymerase

P207B 1 × 1,000u

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: P2077 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

DTT

P117C 1 × 1.25ml100mM View Product

Transcription Optimized 5X Buffer

P118C 1 × 1.25ml View Product

T7 RNA Polymerase

P207E 1 × 5,000u

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: P4074 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

DTT

P117B 1 × 250μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 × 500μl View Product

T7 RNA Polymerase (HC)

P407A 1 × 10,000u

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB
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