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In Vitro Transcription

In vitro transcription products include RNA synthesis systems, nuclear extracts, ribonucleotides, cloning vectors and supporting products for transcription, primer extension and gene expression studies.

Riboprobe® Systems are used to synthesize high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. 

The RiboMAX™ RNA Production Systems generate large quantities of capped or uncapped RNA transcripts suitable for in vitro or in vivo translation reactions. The T7 RiboMAX™ Express Large-Scale RNA Production System is an in vitro transcription system designed for the consistent production of large amounts of RNA in a short amount of time. Milligram amounts of high-quality RNA transcripts up to 14kb have been generated using this kit.

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RNA Synthesis Kit Comparison

 Compare Riboprobe®, RiboMAX™ and RiboMAX™ Express System features and RNA yields.

  Riboprobe® Systems

RiboMAX™ Large Scale RNA Production Systems T7 RiboMAX™ Express Large Scale RNA Production System T7 RiboMAX™ Express RNAi System
Cat.#
P1420, P1430, P1440
P1280, P1300
P1320
P1700
Time
1 hour
2–4 hours
30 minutes
30 minutes
RNA Yield (per 1ml reaction)
Low (100–250µg)
Medium (2–5mg)
High (5–8.5mg)
≥500µg siRNA
≥2mg dsRNA
Radiolabeling
Included Icon
not-available
not-available
not-available
T3 Promoter
Included Icon
not-available
not-available
not-available
SP6 Promoter
Included Icon
Included Icon
not-available
not-available
T7 Promoter
Included Icon
Included Icon
Included Icon
Included Icon
Premixed rNTPs
not-available
not-available
Included Icon
Included Icon
Compatible with Modified Nucleotides
NT
Included Icon
1
NT

NT = not tested.
1 = Compatible, but requires custom order of transcription buffer without nucleotides.

An Introduction to RNA Synthesis Methods

In vitro RNA synthesis methods use bacteriophage DNA-dependent RNA polymerase such as T7, T3 or SP6 RNA polymerase to synthesize RNA from a DNA template. The template DNA for in vitro transcription reactions includes an RNA polymerase promoter upstream of the sequence of interest. The corresponding RNA polymerase is then used to produce synthetic RNA transcripts for use as hybridization probes, as templates for in vitro translation applications, or in structural studies (X-ray crystallography and NMR). Synthesized RNA transcripts are also used for studying cellular RNA functionality in processes such as splicing, RNA processing, intracellular transport, viral infectivity and translation.