Grafting is a horticultural technique commonly used for propagation of woody plants such as fruit trees or roses. Grafting is the combining of the scion, or upper part of a plant, with the rootstock of another plant. The rootstock and scion can be of the same or different species, and will influence certain traits of each other. This allows for particular phenotypic traits, like taste or appearance, to be combined with disease-resistant rootstock to produce a hardier plant.
On a molecular level, mRNAs bind to RNA binding proteins (RBPs) to form ribonucleoprotein (RNP) complexes. This protects the mRNAs as they move over the long distances through graft unions to regulate plant growth and development. To study these RNP complexes in more detail, Wang et al used the T7 RiboMAX™ Express kit to produce biotin-labeled mRNA, which they used to perform electrophoretic mobility-shift assays (EMSAs) with RBPs of interest (4). The researchers concluded that the stability of the RNP complex increases when mRNA binds more than one RNP, and found different combinations of RNPs lead to differences in RNP complex stability. Read the full article here.