Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

QuantiFluor® ONE dsDNA System

Fluorescent Quantitation of dsDNA with "Add-and-Read" Convenience

  • Specific quantitation of dsDNA
  • Increased sensitivity over absorbance at 260nm for low-concentration samples
  • Simple to use and automation-friendly

Size

Catalog number selected: E4871

$ 92.00
Your price:
Please Enquire
This product is discontinued
Request a Sample
Not available in all countries
Add to Helix
QuantiFluor® ONE dsDNA System
100 reactions
$ 92.00
Your price: Log in
Change Configuration

Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.

The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504nm Ex/531nm Em) in an automation-friendly, "Add-and-Read" format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It's as easy to use as NanoDrop® absorbance-based methods, but much more sensitive for low-concentration samples. 

QuantiFluor Nanodrop Spectrophotometer DNA Extraction

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR.  QuantiFluor® ONE dye sensitivity range is 0.2–400ng/µl (1µl sample in 200µl assay).

DNA Extraction & Purification with Better Sensitivity
Sample* Assay

QuantiFluor® ONE dsDNA System

0.2–400ng/µl
0.2–400ng
Qubit® dsDNA HS
0.2–100ng/µl
0.2–100ng
Qubit® dsDNA BR
2–1,000ng/µl
2–1,000ng

*Based on 1µl sample input per assay.


DNA Quantitation Assay Sensitivity and Linearity

The QuantiFluor® ONE System detects as little as 1ng/ml dsDNA per assay using the Quantus™ Fluorometer (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

These graphs show representative QuantiFluor® ONE dsDNA System standard curves generated from Lambda or K562 DNA standards in a 96-well plate format. The assay linearity and dynamic range of either the QuantiFluor® ONE Lambda DNA or Genomic K562 DNA standards are shown from a range of 0.2–400ng/µl (1µl sample in a 200µl assay).

11949ma-w-a

Lambda DNA
11949MA-W-b-2

Easy Setup on Microplate or Single-Tube Fluorometers

System includes all the necessary reagents to quickly set up and quantitate dsDNA and is easy to set up on microplate or single-tube fluorometers, including the following Promega fluorometers.

Quantus Nucleic Acid Quantification Fluorometer

(Use Blue Channel Ex 46nm, Em 515–575nm)


How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wieczorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

Fluorescence vs qPCR

Specifications

You are viewing: E4871 Change Configuration

What's in the box?

Item Part # Size Available Separately

QuantiFluor® ONE dsDNA Dye

E489A 1 × 20ml View Product

QuantiFluor® ONE Lambda DNA

E490B 1 × 80μg

1X TE Buffer (pH 7.5)

E497B 1 × 10ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

EE

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: E4870 Change Configuration

What's in the box?

Item Part # Size Available Separately

QuantiFluor® ONE dsDNA Dye

E489A 5 × 20ml View Product

QuantiFluor® ONE Lambda DNA

E490A 1 × 400μg

1X TE Buffer (pH 7.5)

E497A 1 × 25ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

EE

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Let's find the product that meets your needs.

Talk to a Scientist

Paraj

Paraj

US