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FcγRIIb aAPC/CHO-K1 Cells and aAPC/CHO-K1 Cells

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Measure Potency and Stability of FcγRIIb Crosslinking-Dependent and -Independent Agonist Antibodies

  • Use with ICOS and CD28 bioassays to provide activation of TCR/CD3 complex on effector cells and crosslink antibodies
  • Measure FcγRIIb crosslinking dependence of agonist antibodies that stimulate T cell activation
  • Mechanism-of-action-based

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Catalog number selected: J3252

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FcγRIIb aAPC/CHO-K1 Cells and aAPC/CHO-K1 Cells
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Measure FcγRIIb Crosslinking Dependence of Agonist Antibodies

The antibodies used to stimulate T cell activation via interaction with co-stimulatory receptors on the effector cells can be crosslinked via binding to FcγRIIb receptors on aAPC/CHO-K1 cells. Based on the properties of the antibody tested, the Co-Stimulatory Immune Checkpoint (i.e., ICOS or CD28) bioassays can be conducted in a single genetically engineered cell system or dual genetically engineered cell systems.

FcγRIIb aAPC/CHO-K1 Cells are engineered to express human FcγRIIb and an engineered cell-surface protein designed to activate the TCR complex in an antigen-independent manner. They are available in thaw-and-use or Cell Propagation Model (CPM) formats. The CPM cells can be thawed, propagated and banked for long-term use. These products are not themselves reporter cells; they function to provide activation of the TCR/CD3 complex on effector cells and crosslink anti-CD28 and anti-ICOS agonist antibodies.

aAPC/CHO-K1 Cells express an engineered cell-surface protein designed to activate the TCR complex in an antigen-independent manner. They are available in thaw-and-use or Cell Propagation Model (CPM) formats. The CPM cells can be thawed, propagated and banked for long-term use. These products are not themselves reporter cells; they function to provide activation of the TCR/CD3 complex on effector cells. These cells do not express FcγRIIb.

  • FcγRIIb aAPC/CHO-K1 Cells (Cat.# JA9331, JA9335)
  • FcγRIIb aAPC/CHO-K1 Cells, Propagation Model (Cat.# J3252)
  • aAPC/CHO-K1 Cells (Cat.# JA9441, JA9445)
  • aAPC/CHO-K1 Cells, Propagation Model (Cat.# J3312)
  • Control Ab, Anti-CD28 (Cat.# K1231), is available separately
  • Control Ab, Anti-ICOS (Cat.# K1241), is available separately

Assay Principle

The ICOS Bioassay measures the potency and stability of ligands or agonist antibodies and other biologics that bind and activate ICOS.

Representation of the ICOS Bioassay.

Representation of the ICOS Bioassay. The ICOS Bioassay for FcγRIIb-dependent agonist antibodies consists of two cell lines, ICOS Effector Cells and FcγRIIb aAPC/CHO-K1 Cells. In the absence of agonist antibody, ICOS is not activated and the luminescence signal is low. In the presence of FcγRIIb aAPC/CHO-K1 Cells, the anti-ICOS antibody can be crosslinked, thereby inducing ICOS pathway-activating luminescence in a dose-dependent manner. ICOS Effector Cells can also be used with antibodies that are FcγRIIb-independent by substituting aAPC/CHO-K1 Cells.

The CD28 Bioassay measures the potency and stability of ligands or agonist antibodies and other biologics that bind and activate CD28.

Representation of the CD28 Bioassay.

Representation of the CD28 Bioassay. The CD28 Bioassay for FcγRIIb-dependent agonist antibodies consists of two cell lines, CD28 Effector Cells and FcγRIIb aAPC/CHO-K1 Cells. In the absence of agonist antibody, CD28 is not activated and the luminescence signal is low. In the presence of FcγRIIb aAPC/CHO-K1 Cells, the anti-CD28 antibody can be crosslinked, thereby enhancing induction of CD28 pathway-activating luminescence in a dose-dependent manner. CD28 Effector Cells can also be used with antibodies that are FcγRIIb-independent by substituting aAPC/CHO-K1 Cells.

Specifications

You are viewing: J3252 Change Configuration

What's in the box?

Item Part # Size

FcγRIIb aAPC/CHO-K1 (CPM)

J325A 2 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use luminescent assay reagents purchased from Promega for all luminescence-based assays using this product, or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Specifications

You are viewing: JA9331 Change Configuration

What's in the box?

Item Part # Size

FcγRIIb aAPC/CHO-K1 Cells

J326A 1 × 0.5ml

Ham's F-12 Medium

J123A 1 × 25ml

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: JA9335 Change Configuration

What's in the box?

Item Part # Size

FcγRIIb aAPC/CHO-K1 Cells

J326A 5 × 0.5ml

Ham's F-12 Medium

J123A 5 × 25ml

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: J3312 Change Configuration

What's in the box?

Item Part # Size

aAPC/CHO-K1 Cells (CPM)

J119B 2 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use luminescent assay reagents purchased from Promega for all luminescence-based assays using this product, or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Specifications

You are viewing: JA9441 Change Configuration

What's in the box?

Item Part # Size

aAPC/CHO-K1 Cells

J119A 1 × 0.5ml

Ham's F-12 Medium

J123A 1 × 25ml

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: JA9445 Change Configuration

What's in the box?

Item Part # Size

aAPC/CHO-K1 Cells

J119A 5 × 0.5ml

Ham's F-12 Medium

J123A 5 × 25ml

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

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