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FcγRIIb aAPC/CHO-K1 Cells and aAPC/CHO-K1 Cells

Measure Potency and Stability of FcγRIIb Crosslinking-Dependent and -Independent Agonist Antibodies

  • Use with ICOS and CD28 bioassays to provide activation of TCR/CD3 complex on effector cells and crosslink antibodies
  • Measure FcγRIIb crosslinking dependence of agonist antibodies that stimulate T cell activation
  • Mechanism-of-action-based

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Catalog number selected: J3252

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FcγRIIb aAPC/CHO-K1 Cells and aAPC/CHO-K1 Cells
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Measure FcγRIIb Crosslinking Dependence of Agonist Antibodies

The antibodies used to stimulate T cell activation via interaction with co-stimulatory receptors on the effector cells can be crosslinked via binding to FcγRIIb receptors on aAPC/CHO-K1 cells. Based on the properties of the antibody tested, the Co-Stimulatory Immune Checkpoint (i.e., ICOS or CD28) bioassays can be conducted in a single genetically engineered cell system or dual genetically engineered cell systems.

FcγRIIb aAPC/CHO-K1 Cells are engineered to express human FcγRIIb and aAPC. They are available in thaw-and-use or Cell Propagation Model (CPM) formats. The CPM cells can be thawed, propagated and banked for long-term use. These products are not themselves reporter cells; they function to provide activation of the TCR/CD3 complex on effector cells and crosslink anti-CD28 and anti-ICOS agonist antibodies.

aAPC/CHO-K1 Cells are engineered to express aAPC. They are available in thaw-and-use or Cell Propagation Model (CPM) formats. The CPM cells can be thawed, propagated and banked for long-term use. These products are not themselves reporter cells; they function to provide activation of the TCR/CD3 complex on effector cells. These cells do not express FcγRIIb.

  • FcγRIIb aAPC/CHO-K1 Cells (Cat.# JA9331, JA9335)
  • FcγRIIb aAPC/CHO-K1 Cells, Propagation Model (Cat.# J3252)
  • aAPC/CHO-K1 Cells (Cat.# JA9441, JA9445)
  • aAPC/CHO-K1 Cells, Propagation Model (Cat.# J3312)
  • Control Ab, Anti-CD28 (Cat.# K1231), is available separately
  • Control Ab, Anti-ICOS (Cat.# K1241), is available separately

Assay Principle

The ICOS Bioassay measures the potency and stability of ligands or agonist antibodies and other biologics that bind and activate ICOS.

Representation of the ICOS Bioassay.

Representation of the ICOS Bioassay. The ICOS Bioassay for FcγRIIb-dependent agonist antibodies consists of two cell lines, ICOS Effector Cells and FcγRIIb aAPC/CHO-K1 Cells. In the absence of agonist antibody, ICOS is not activated and the luminescence signal is low. In the presence of FcγRIIb aAPC/CHO-K1 Cells, the anti-ICOS antibody can be crosslinked, thereby inducing ICOS pathway-activating luminescence in a dose-dependent manner. ICOS Effector Cells can also be used with antibodies that are FcγRIIb-independent by substituting aAPC/CHO-K1 Cells.

The CD28 Bioassay measures the potency and stability of ligands or agonist antibodies and other biologics that bind and activate CD28.

Representation of the CD28 Bioassay.

Representation of the CD28 Bioassay. The CD28 Bioassay for FcγRIIb-dependent agonist antibodies consists of two cell lines, CD28 Effector Cells and FcγRIIb aAPC/CHO-K1 Cells. In the absence of agonist antibody, CD28 is not activated and the luminescence signal is low. In the presence of FcγRIIb aAPC/CHO-K1 Cells, the anti-CD28 antibody can be crosslinked, thereby enhancing induction of CD28 pathway-activating luminescence in a dose-dependent manner. CD28 Effector Cells can also be used with antibodies that are FcγRIIb-independent by substituting aAPC/CHO-K1 Cells.

Specifications

You are viewing: J3252 Change Configuration

What's in the box?

Item Part # Size

FcγRIIb aAPC/CHO-K1 (CPM)

J325A 2 × 1ml

SDS

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Download SDSPDF (265 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Specifications

You are viewing: JA9331 Change Configuration

What's in the box?

Item Part # Size

FcγRIIb aAPC/CHO-K1 Cells

J326A 1 × 0.5ml

Ham's F-12 Medium

J123A 1 × 25ml

SDS

Choose language:

Download SDSPDF (287 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: JA9335 Change Configuration

What's in the box?

Item Part # Size

FcγRIIb aAPC/CHO-K1 Cells

J326A 5 × 0.5ml

Ham's F-12 Medium

J123A 5 × 25ml

SDS

Choose language:

Download SDSPDF (287 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: J3312 Change Configuration

What's in the box?

Item Part # Size

aAPC/CHO-K1 Cells (CPM)

J119B 2 × 1ml

SDS

Choose language:

Download SDSPDF (203 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Specifications

You are viewing: JA9441 Change Configuration

What's in the box?

Item Part # Size

aAPC/CHO-K1 Cells

J119A 1 × 0.5ml

Ham's F-12 Medium

J123A 1 × 25ml

SDS

Choose language:

Download SDSPDF (224 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: JA9445 Change Configuration

What's in the box?

Item Part # Size

aAPC/CHO-K1 Cells

J119A 5 × 0.5ml

Ham's F-12 Medium

J123A 5 × 25ml

SDS

Choose language:

Download SDSPDF (225 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

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