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Protease Inhibitor Cocktail

Protease Inhibitor Cocktail 50X 1ml
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Prevents Protein Degradation After Cell Lysis

  • Effective against many proteases
  • Best-in-class protease inhibition
  • Works with a wide array of fusion tags (e.g., Flag®, His tag, GST) and capture technologies
  • Freeze-dried powder requires resuspension in 1ml of 100% ethanol or DMSO

Size

Catalog number selected: G6521

$ 91.00
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Protease Inhibitor Cocktail
1ml
$ 91.00
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Best-in-Class Protease Inhibition

Prevent protein degradation after cell lysis. This mixture of six different inhibitors covers a wide range of target proteases. The inhibitor cocktail is EDTA-free and provided as a powder, ready for reconstitution in 1ml of either 100% ethanol or DMSO to obtain a 50X working solution.

Protease Inhibitor Cocktail provides the best-in-class level of protease inhibition. It works with a wide array of protein fusion tags (e.g., Flag®, His tag, GST tag) and capture technologies and is ideally suited for HaloTag® Technology-based approaches.

Applications

  • Protein purification from mammalian cell culture.
  • Protein purification from insect cell culture.
  • Analysis and capture of protein:protein, protein:DNA interactions.

References

  1. Ohana, R.F. et al. (2011) HaloTag-based purification of functional human kinases from mammalian cells. Protein Expr. Purif. 76, 154–64.
  2. Lee, J.-S. et al. (2011) Molecular and functional characterization of aryl hydrocarbon receptor repressor from the chicken (Gallus gallus): Interspecies similarities and differences. Toxicol. Sci. 119, 319–34.
  3. Nakashima, R. et al. (2010) The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody. Rheumatology 49, 433–40.
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*Assay performed with 43fmol of purified trypsin. cOmplete is a trademark of Hoffmann-La Roche. BaculoGold is a trademark of Becton, Dickinson and Company.

Specifications

What's in the box?

Item Part # Size

Protease Inhibitor Cocktail, 50X

G652A 1 × 1ml resuspended

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

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