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MagneGST™ Protein Purification System

Simple Method for Glutathione-S-Transferase Fusion Protein Purification

  • One-step purification of multiple samples; no need for high-speed centrifugation after cell lysis
  • Use with 1–50ml of cell culture
  • Achieve high yields with little to no nonspecific background

Size

Catalog number selected: V8600

$ 199.00 Your price: Log In

MagneGST™ Protein Purification System
40 reactions
$ 199.00
Your price: Log In
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One-Step Protein Purification from 1–50ml of Culture

The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST™ Particles) are used to isolate GST-tagged protein directly from a crude or cleared lysate using either a manual or automated procedure and requires use of a magnetic stand. GST-tagged proteins can be purified on a small scale from 1ml of culture or on a large scale using more than 50ml of culture. Samples also can be processed using a robotic platform. MagneGST™ particles are supplied as a 25% slurry and have a binding capacity of 5–10mg of GST protein per 1ml of settled resin.

The benefits of this system include one-step purification of multiple samples with easy handling, no need for lysate clearing and a scalable system that can be used with 1–50ml of cell culture.

For more information, see the Protocols & Applications Guide.

Note: This product requires the use of a magnetic stand that is not included. 

Applications

  • Protein purification of glutathione-S-transferase fusion proteins from cleared or crude E. coli cell lysate.
  • High-throughput purification of GST-tagged proteins using automated platforms.

 

4110TB.eps
Purification of GST-ubiquitin and GST-luciferase.

Protocols

Specifications

You are viewing: 40 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

RQ1 RNase-Free DNase

M610A 1 x 1,000u View Product

MagneGST™ Cell Lysis Reagent

V481A 1 x 12ml

MagneGST™ Glutathione Particles

V861A 1 x 4ml View Product

MagneGST™ Binding/Wash Buffer

V870A 1 x 80ml

Glutathione

V871A 1 x 2ml

Tris Buffer, 0.5M (pH 8.1)

V872A 1 x 2ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Specifications

You are viewing: 200 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

RQ1 RNase-Free DNase

M610A 1 x 1,000u View Product

MagneGST™ Cell Lysis Reagent

V481B 1 x 60ml

MagneGST™ Glutathione Particles

V861B 2 x 10ml View Product

MagneGST™ Binding/Wash Buffer

V870B 2 x 200ml

Glutathione

V871B 1 x 10ml

Tris Buffer, 0.5M (pH 8.1)

V872B 1 x 10ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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