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FastBreak™ Cell Lysis Reagent

Quick E. coli Cell Culture Lysis, No Centrifugation or Mechanical Disruption Required

  • Eliminate centrifugation and mechanical disruption
  • Simple, add-and-incubate protocol
  • Compatible with manual or robotic platforms

Size

Catalog number selected: V8571

$ 39.00 Your price: Log In

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FastBreak™ Cell Lysis Reagent
15ml
$ 39.00
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Efficient Lysis of E. coli Cultures; No Centrifugation or Mechanical Disruption Needed

FastBreak™ Cell Lysis Reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein  released. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis™ Protein Purification System. This product is suitable for both manual and automated protocols. 

Eliminate centrifugation and mechanical disruption with this easy-to-use, add and incubate reagent, compatible with manual and robotic platforms.

Manual purification of a 6X His-tagged fusion protein using FastBreak™ Cell Lysis Reagent and the MagneHis™ Protein Purification System.

Manual purification of a 6X His-tagged fusion protein using FastBreak™ Cell Lysis Reagent and the MagneHis™ Protein Purification System. Lane 1, bacterial cell lysate expressing 6X His-tagged firefly luciferase lysed by the addition of FastBreak™ Reagent; lane 2, postlysis cell supernatant demonstrating that the protein is solubilized; lane 3, flowthrough of cell lysate after the addition of MagneHis™ Ni-Particles; lane 4, eluted 6X His-tagged firefly luciferase; lane 5, Broad Range Protein Molecular Weight Markers (Cat.# V8491).

Specifications

You are viewing: V8571 Change Configuration

What's in the box?

Item Part # Size

FastBreak™ Cell Lysis Reagent, 10X

V857A 1 × 15ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

P

Patents and Disclaimers

Patent Pending.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Specifications

You are viewing: V8572 Change Configuration

What's in the box?

Item Part # Size

FastBreak™ Cell Lysis Reagent, 10X

V857B 1 × 60ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

P

Patents and Disclaimers

Patent Pending.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Specifications

You are viewing: V8573 Change Configuration

What's in the box?

Item Part # Size

FastBreak™ Cell Lysis Reagent, 10X

V857C 1 × 100ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

P

Patents and Disclaimers

Patent Pending.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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