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SARS-CoV-2 RT-qPCR Kits for Wastewater

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Convenient Kits for Quantifying SARS-CoV-2 in Wastewater

  • Built-in controls to ensure consistent results
  • Designed for wastewater surveillance programs using CDC-recommended primer/probe sets and controls
  • Include quantification standard RNAs for SARS-CoV-2 N+E gene and PMMoV

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Catalog number selected: AM2100

$ 945.00
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SARS-CoV-2 RT-qPCR Kits for Wastewater
N1, N2 or E/200 reactions
$ 945.00
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Sensitive Quantification of SARS-CoV-2 Viral RNA in Wastewater

Detect SARS-CoV-2 viral RNA in preprocessed wastewater with high efficiency and sensitivity using the SARS-CoV-2 RT-qPCR Kit for Wastewater. The master mix allows sensitive detection and quantification even in the presence of reverse transcriptase inhibitors or PCR inhibitors common in wastewater samples, such as humic acids.

The kits contain a combination of primer/probe sets and controls recommended by the US Centers for Disease Control (CDC). This includes primer/probe sets targeting the N1 or N2 regions of the nucleocapsid gene, or the envelope (E) gene; primers detecting Pepper Mild Mottle Virus (PMMoV) as an internal process control; and an internal amplification control.

Additional RT-qPCR kits for detecting MS2, HF183/crAssphage, norovirus, enterovirus and other pathogens are also under development. Please contact applied@promega.com for more information.

Select Which Product is Right for You

The links in the table for Cat.# starting with AM will take you to the ordering information for the selected product at the top of this page.

Cat.# starting with CS are part of our Early Access program with limited supply and available upon request. Please contact your Promega representative or submit the form behind the links in the table for the Cat.# starting with CS to learn about availability.

Learn more about wastewater surveillance methods and tools.

Cat.# Name Size Assay Target(s) Quant Standard Process and Normalization Control
AM2150 GoTaq® Enviro Wastewater SARS-CoV-2 System, N1/N2 200 reactions/kit N1, N2 and PMMoV N+E and PMMoV RNA IAC and PMMoV
AM2160 GoTaq® Enviro Wastewater SARS-CoV-2 System, E/N2 200 reactions/kit E, N2 and PMMoV N+E and PMMoV RNA IAC and PMMoV
AM2110 GoTaq® Enviro Wastewater SARS-CoV-2 System, N1 200 reactions/kit N1 and PMMoV N+E and PMMoV RNA IAC and PMMoV
AM2120 GoTaq® Enviro Wastewater SARS-CoV-2 System, N2 200 reactions/kit N2 and PMMoV N+E and PMMoV RNA IAC and PMMoV
AM2130 GoTaq® Enviro Wastewater SARS-CoV-2 System, E 200 reactions/kit E and PMMoV N+E and PMMoV RNA IAC and PMMoV
AM2100 GoTaq® Enviro Wastewater SARS-CoV-2 System, N1/N2/E 200 reactions/kit N1, N2 or E and PMMoV N+E and PMMoV RNA IAC and PMMoV
AM2140 GoTaq® Enviro PMMoV Quant Kit, Quasar® 670 100 reactions/kit PMMoV PMMoV RNA
AM2050 SARS-CoV-2 (N+E) RNA 100µl SARS-CoV-2 RNA
AM2060 SARS-CoV-2 (N+E) dsDNA 100µl SARS-CoV-2 DNA
AM2070 PMMoV RNA 100µl PMMoV RNA
CS317419 GoTaq® Enviro HF183/crAssphage Quant Kit 100 reactions/kit crAssphage and HF183 DNA IPC
CS317420 GoTaq® Enviro Noro/HepA RT-qPCR System 100 reactions/kit Norovirus GI, Norovirus GII and Hepatitis A Virus DNA
CS317421 GoTaq® Enviro FluA/FluB/SARS-CoV-2/RSV 100 reactions/kit Influenza A, Influenza B, SARS-CoV-2 “SC2” and RSV (A+B) DNA
CS317423 GoTaq® Enviro Candida auris qPCR System 100 reactions/kit Candida auris FSK1 and HF183 DNA
CS346201 ZDC 200 reactions/kit Zika Virus, Chikungunya Virus, Dengue Virus Available separately upon request RNase P
CS317417 MS2 RT-qPCR Kit 100 reactions/kit MS2 MS2 DNA

How Wastewater Testing Can Help Campuses Reopen

Robert Brooks is the Technical Director and Operations Manager at Microbac Laboratories in Oak Ridge, Tennessee. Since the start of the pandemic, his lab has begun developing methods for environmental testing of the SARS-CoV-2 virus using PCR analysis. They based their PCR tests on primer sets used for clinical testing recommended by the CDC, but they had to add other components. “You can’t just take a clinical test and start running environmental samples. It doesn’t quite work like that.”, says Robert. The internal controls in clinical testing kits were meant for human samples, not wastewater. To ensure accurate results, they needed a kit specifically designed for wastewater.

Learn more about how Microbac Laboratories is using the SARS-CoV-2 RT-qPCR Kit for Wastewater to develop low-cost SARS-CoV-2 surveillance testing for university campuses in this blog.

“The Promega kits include internal controls for all the different parameters that need to be measured. The separate targets give us a lot of flexibility, which is what we need."
Robert Brooks, Technical Director and Operations Manager, Microbac Laboratories

Specifications

You are viewing: AM2100 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

N1 and PMMoV Primer/Probe/IAC Mix, 20X

AM211A 1 × 100μl

N2 and PMMoV Primer/Probe/IAC Mix, 20X

AM212A 1 × 100μl

E and PMMoV Primer/Probe/IAC Mix, 20X

AM213A 1 × 100μl

Nuclease-Free Water

P119A 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2110 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

N1 and PMMoV Primer/Probe/IAC Mix, 20X

AM211A 2 × 100μl

Nuclease-Free Water

P119A 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2120 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

N2 and PMMoV Primer/Probe/IAC Mix, 20X

AM212A 2 × 100μl

Nuclease-Free Water

P119A 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2130 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

E and PMMoV Primer/Probe/IAC Mix, 20X

AM213A 2 × 100μl

Nuclease-Free Water

P119A 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2140 Change Configuration

What's in the box?

Item Part # Size Available Separately

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 1 × 1ml

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

PMMoV Primer/Probe Mix, Quasar® 670

AM214A 1 × 100μl

CXR Reference Dye

C541A 1 × 100μl View Product

Nuclease-Free Water

P119A 1 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2070 Change Configuration

What's in the box?

Item Part # Size

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: AM2050 Change Configuration

What's in the box?

Item Part # Size

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: AM2060 Change Configuration

What's in the box?

Item Part # Size

SARS-CoV-2 (N+E) dsDNA, 2 × 106 copies/µl

AM206A 1 × 100μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: AM2150 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

N1/N2 & PMMoV Primer/Probe/IAC Mix, 20X

AM215A 2 × 100μl

Nuclease-Free Water

P119A 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2160 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

E/N2 & PMMoV Primer/Probe/IAC Mix, 20X

AM216A 2 × 100μl

Nuclease-Free Water

P119A 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

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