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Viral RNA/DNA Concentration and Extraction Kits for Wastewater

Direct Capture and Purification of Viral DNA/RNA From Wastewater

  • Process 40ml input sample volume of wastewater
  • Extract TNA in 2 hours or less
  • Efficiently remove PCR inhibitors
  • Choose from three formats to fit your sample throughput: Manual (A2991); Maxwell® automation (AS1831); or high-throughput, plate-based automation (AX9190)

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Catalog number selected: A2991

$ 360.00
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Viral RNA/DNA Concentration and Extraction Kits for Wastewater
Wizard® Enviro TNA Kit/25 preps
$ 360.00
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Direct Capture of SARS-CoV-2 RNA in 2 Hours

The Enviro Total Nucleic Acid Kits for Wastewater include reagents and consumables to concentrate and purify total nucleic acids (TNA) in one convenient kit. Our unique vacuum-based direct capture method simplifies sample processing by concentrating and capturing viral TNA quicker than precipitation methods without the use of cumbersome ultracentrifugation, while achieving high and consistent yield. The entire process from sample to purified TNA takes less than 2 hours and is scalable and adaptable to your needs. The process also removes most PCR inhibitors, so the resulting TNA can be used directly for amplification of SARS-CoV-2 RNA using the SARS-CoV-2 RT-qPCR Kit for Wastewater.

We offer three kit formats based on sample throughput. Direct capture of nucleic acids is followed by purification using mini spin columns or benchtop automation using Maxwell® Instruments. A plate-based high-throughput kit is also available. An optional step can be included to extract nucleic acids from solid samples. 

What Customers Are Saying


“We are very happy to have implemented the large volume extraction kits in our analysis of wastewater for SARS-CoV-2. The integration of concentration and extraction steps has been instrumental in expediting our workflow. The kits also provide higher reproducibility between biological replicates compared to the method we were using previously.”


Jeseth Delgado Vela
Asst. Professor, Howard University


“We have tested the new Promega Wastewater Kit and were very pleased with results. In my side-by side comparison, the Promega Wastewater Kit has demonstrated way higher viral RNA recovery. We are planning to use it for all future wastewater work.”


Sergey Balashov
PCR Laboratory Director, ESML Analytical

Simple Direct Capture Protocol

The filter-based Direct Capture method allows rapid purification and concentration of viral nucleic acids from large volumes of wastewater samples.

17691mb

Choose A Format For Your Sample Throughput

Manual

The Wizard® Enviro Total Nucleic Acid Kit (A2991) allows you to get purified TNA in 90 minutes or less with a simple protocol. First, the TNA is directly captured from wastewater using the PureYield™ Midicolumn and Vac-Man® Laboratory Vacuum Manifold. Then, the lysate is further cleaned using PureYield™ Minicolumns. The recommended protocol starts from 40ml of wastewater and results in 40µl of TNA. For more details, view the protocol video.

Maxwell® Automation

The Maxwell® RSC Enviro Total Nucleic Acid Kit (AS1831) allows you to get purified TNA in 2 hours with vacuum-based direct capture of TNA followed by automated TNA extraction using Maxwell® Instruments. The Maxwell® RSC Instrument can process 1 to 16 samples, and the Maxwell® RSC 48 Instrument can process 1 to 48 samples in a single run. Viral TNA is first captured from wastewater using the PureYield™ Midicolumn and Vac-Man® Laboratory Vacuum Manifold. Then, the lysate is loaded into the Maxwell® RSC Instrument for walkaway RNA extraction. The recommended protocol starts from 40ml of wastewater and results in 40µl of TNA. For more details, view the protocol video.

Need a high-throughput solution?

The Maxwell® HT Environmental TNA Kit (AX9190) can be used for high-throughput, automated extraction of viral TNA from wastewater. Samples are processed in 96-well plates using magnetic separation. The kit can be used with any automated high-throughput platform. Our Field Support Scientists can help you implement or optimize automation of viral RNA extraction methods that meets your specific needs. Please contact us for more information.

Direct Capture Results in Higher Yield Than PEG/NaCl

wastewater concentration SARS-CoV-2 RNA yield comparison

Preliminary data shows the direct capture method resulted in 30 times higher SARS-CoV-2 RNA yield compared to PEG/NaCl. Wastewater samples were collected from a major US city.

Have questions? We're here to help!
 

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Specifications

You are viewing: A2991 Change Configuration

What's in the box?

Item Part # Size Available Separately

Binding Buffer 1 (BBD)

A2981 1 × 320ml

Binding Buffer 2 (BBE)

MC1501 1 × 30ml

Protease Solution

A144C 1 × 13ml

Column Wash 1 (CWE)

A296A 1 × 85.3ml

Column Wash 2 (RWA)

A297A 1 × 206ml

Nuclease-Free Water

P1195 1 × 150ml View Product

PureYield™ Binding Columns

A245A 5 × 5 each

PureYield™ Minicolumns

A247C 1 × 25/pk

PureYield™ Collection Tubes

A167C 1 × 25/pk

Elution Tubes

Z200C 1 × 50/pk

Reservoir Extension Funnel

A3041 1 × 25/pk

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: AS1831 Change Configuration

What's in the box?

Item Part # Size Available Separately

Column Wash 1 (CWE)

A296A 2 × 85.3ml

Column Wash 2 (RWA)

A297A 2 × 206ml

Nuclease-Free Water

P1195 1 × 150ml View Product

PureYield™ Binding Columns

A245C 12 × 4 each

Elution Tubes (0.5ml)

AS620A 1 × 50/pk View Product

Maxwell® RSC Cartridge (RSCJ)

AS159A 48 × 1 each

RSC Plungers

AS1670 1 × 48/pack View Product

Elution Tubes

Z200C 1 × 50/pk

Binding Buffer 1 (BBD)

A2981 2 × 320ml

Binding Buffer 2 (BBE)

MC1501 2 × 30ml

Protease Solution

A1442 1 × 30ml

Reservoir Extension Funnel

A3041 2 × 25/pk

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. No. 7,329,488 and S. Korean Pat. No. 100483684.

Specifications

You are viewing: A2981 Change Configuration

SDS

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Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: MC1501 Change Configuration

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: A1442 Change Configuration

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: A8281 Change Configuration

What's in the box?

Item Part # Size

Elution Buffer

A828B 1 × 50ml

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

DD

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