SARS-CoV-2 RT-qPCR Kits for Wastewater

N1, N2 and E/200 reactions

$ 900.00

Your price: Log in

Change Configuration

Sensitive Quantification of SARS-CoV-2 Viral RNA in Wastewater

Detect SARS-CoV-2 viral RNA in preprocessed wastewater with high efficiency and sensitivity using the SARS-CoV-2 RT-qPCR Kit for Wastewater. The master mix allows sensitive detection and quantification even in the presence of reverse transcriptase inhibitors or PCR inhibitors common in wastewater samples, such as humic acids.

The kits contain a combination of primer/probe sets and controls recommended by the US Centers for Disease Control (CDC). This includes primer/probe sets targeting the N1 or N2 regions of the nucleocapsid gene, or the envelope (E) gene; primers detecting Pepper Mild Mottle Virus (PMMoV) as an internal process control; and an internal amplification control.

Additional RT-qPCR kits for detecting MS2, HF183/crAssphage, norovirus, enterovirus and other pathogens are also under development. Please contact applied@promega.com for more information.

New! Detect Common SARS-CoV-2 Variants

As the SARS-CoV-2 virus continues to mutate and generate new variants of concern, it is important to monitor the spread of these variants with wastewater surveillance. The SARS-CoV-2 Variant Panel contains eight primer/probe mixes (Assays A-H) that detect mutations common to the Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617), Kappa (B.1.617.1) and Omicron (B.1.1.529) strains. The master mix (GoTaq® Enviro RT-qPCR System; AM2010) is available separately.

See how the SARS-CoV-2 Kits for Wastewater are being used in Karthikeyan, S. et al. Wastewater Sequencing Uncovers Early, Cryptic SARS-CoV-2 Variant Transmission.

Assay	Alpha, V1 (B.1.1.7)	Beta, V2 (B.1.351)	Gamma, V3 (P.1)	Delta (B.1.617.2)	Kappa (B.1.617.1)	Omicron (BA.1)	Omicron (BA.2)	Omicron (BA.4&5)	Omicron (BA.2.12.1)
A	S:N501Y	S:N501Y	S:N501Y	WT	WT	S:N501Y	S:N501Y	S:N501Y	S:N501Y
B	S:H69-/V70-	WT	WT	WT	WT	S:H69-/V70-	WT	S:H69-/V70-	WT
C	WT	S:K417N	No Signal	WT	WT	S:K417N	S:K417N	S:K417N	S:K417N
D	WT	No Signal	S:K417T	WT	WT	No Signal	No Signal	No Signal	No Signal
E	S:Y144-	WT	WT	WT	WT	S:Y144-	WT	WT	WT
F	WT	S:E484K	S:E484K	WT	S:E484Q	S:E484A	S:E484A	S:E484A	S:E484A
G	S:P681H	WT	WT	S:P681R	S:P681R	S:P681H	S:P681H	S:P681H	S:P681H
H	WT	WT	WT	S:L452R	S:L452R	WT	WT	S:L452R	S:L452Q

Key

Signal in FAM

Signal in HEX

No Signal, Other Mutation

Reference: https://covariants.org/shared-mutations

The Variant Panel kit contains sufficient material for 8 × 50 reactions (20μl RT-qPCR reaction size) or 8 × 100 reactions (10μl RT-qPCR reaction size).

Select Which Product is Right for You

Learn more about wastewater surveillance methods and tools.

Cat.#	Name	Reactions/Kit	Gene(s)	Quant Standard	Process & Normalization Control
AM2100	GoTaq® Enviro Wastewater SARS-CoV-2 System, N1/N2/E	200	N1, N2 or E	N&E and PMMoV RNA	IAC & PMMoV
AM2110	GoTaq® Enviro Wastewater SARS-CoV-2 System, N1	200	N1	N&E and PMMoV RNA	IAC & PMMoV
AM2120	GoTaq® Enviro Wastewater SARS-CoV-2 System, N2	200	N2	N&E and PMMoV RNA	IAC & PMMoV
AM2130	GoTaq® Enviro Wastewater SARS-CoV-2 System, E	200	E	N&E and PMMoV RNA	IAC & PMMoV
CS3174B02	SARS-CoV-2 Variant Panel- 8 Targets	8 × 50 preps	8 sites on S	None
AM2140	GoTaq® Enviro PMMoV Quant Kit, Quasar® 670	100	PMMoV	PMMoV RNA
CS317417	MS2 RT-qPCR Kit	100	MS2	MS2 DNA
AM2070	PMMoV RNA	100µl
AM2050	SARS-CoV-2 (N+E) RNA	100µl
AM2060	SARS-CoV-2 (N+E) dsDNA	100µl

How Wastewater Testing Can Help Campuses Reopen

Robert Brooks is the Technical Director and Operations Manager at Microbac Laboratories in Oak Ridge, Tennessee. Since the start of the pandemic, his lab has begun developing methods for environmental testing of the SARS-CoV-2 virus using PCR analysis. They based their PCR tests on primer sets used for clinical testing recommended by the CDC, but they had to add other components. “You can’t just take a clinical test and start running environmental samples. It doesn’t quite work like that,” says Robert. The internal controls in clinical testing kits were meant for human samples, not wastewater. To ensure accurate results, they needed a kit specifically designed for wastewater.

Learn more about how Microbac Laboratories is using the SARS-CoV-2 RT-qPCR Kit for Wastewater to develop low-cost SARS-CoV-2 surveillance testing for university campuses in this blog.

“The Promega kits include internal controls for all the different parameters that need to be measured. The separate targets give us a lot of flexibility, which is what we need."

Robert Brooks, Technical Director and Operations Manager, Microbac Laboratories

Protocols

Complete Protocol

GoTaq® Enviro Wastewater SARS-CoV-2 Systems Technical ManualPDF (1490 KB) – English

GoTaq® Enviro PMMoV Quant Kit, Quasar® 670 Technical ManualPDF (1270 KB) – English

SARS-CoV-2 (N+E) RNA Quant Standard Product InformationPDF (114 KB) – English

SARS-CoV-2 (N+E) dsDNA Quant Standard Product InformationPDF (103 KB) – English

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
N1 and PMMoV Primer/Probe/IAC Mix, 20X	AM211A	1 × 100μl
N2 and PMMoV Primer/Probe/IAC Mix, 20X	AM212A	1 × 100μl
E and PMMoV Primer/Probe/IAC Mix, 20X	AM213A	1 × 100μl
Nuclease-Free Water	P119A	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
N1 and PMMoV Primer/Probe/IAC Mix, 20X	AM211A	2 × 100μl
Nuclease-Free Water	P119A	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
N2 and PMMoV Primer/Probe/IAC Mix, 20X	AM212A	2 × 100μl
Nuclease-Free Water	P119A	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
E and PMMoV Primer/Probe/IAC Mix, 20X	AM213A	2 × 100μl
Nuclease-Free Water	P119A	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Storage Conditions

What's in the box?

Item	Part #	Size	Available Separately
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	1 × 1ml
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
PMMoV Primer/Probe Mix, Quasar® 670	AM214A	1 × 100μl
CXR Reference Dye	C541A	1 × 100μl	View Product
Nuclease-Free Water	P119A	1 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Storage Conditions

What's in the box?

Item	Part #	Size
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size
SARS-CoV-2 (N+E) dsDNA, 2 × 10⁶ copies/µl	AM206A	1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Promega's Cookie Policy

SARS-CoV-2 RT-qPCR Kits for Wastewater

Convenient Kits for Quantifying SARS-CoV-2 in Wastewater

Sensitive Quantification of SARS-CoV-2 Viral RNA in Wastewater

New! Detect Common SARS-CoV-2 Variants

Select Which Product is Right for You

How Wastewater Testing Can Help Campuses Reopen

Protocols

Complete Protocol

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

SDS

Certificate of Analysis

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

SDS

Certificate of Analysis

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Resources

Articles

Other Resources

Similar Products

GoTaq® Enviro qPCR and RT-qPCR Systems

Frequently Used With

Viral RNA/DNA Concentration and Extraction Kits for Wastewater

Americas

Pacific Asia

Europe