SARS-CoV-2 RT-qPCR Kits for Wastewater

N1, N2 or E/200 reactions

$ 900.00

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Sensitive Quantification of SARS-CoV-2 Viral RNA in Wastewater

Detect SARS-CoV-2 viral RNA in preprocessed wastewater with high efficiency and sensitivity using the SARS-CoV-2 RT-qPCR Kit for Wastewater. The master mix allows sensitive detection and quantification even in the presence of reverse transcriptase inhibitors or PCR inhibitors common in wastewater samples, such as humic acids.

The kits contain a combination of primer/probe sets and controls recommended by the US Centers for Disease Control (CDC). This includes primer/probe sets targeting the N1 or N2 regions of the nucleocapsid gene, or the envelope (E) gene; primers detecting Pepper Mild Mottle Virus (PMMoV) as an internal process control; and an internal amplification control.

Additional RT-qPCR kits for detecting MS2, HF183/crAssphage, norovirus, enterovirus and other pathogens are also under development. Please contact applied@promega.com for more information.

Select Which Product is Right for You

The links in the table for Cat.# starting with AM will take you to the ordering information for the selected product at the top of this page.

Cat.# starting with CS are part of our Early Access program with limited supply and available upon request. Please contact your Promega representative or submit the form behind the links in the table for the Cat.# starting with CS to learn about availability.

Learn more about wastewater surveillance methods and tools.

Cat.#	Name	Size	Assay Target(s)	Quant Standard	Process and Normalization Control
AM2150	GoTaq® Enviro Wastewater SARS-CoV-2 System, N1/N2	200 reactions/kit	N1, N2 and PMMoV	N+E and PMMoV RNA	IAC and PMMoV
AM2160	GoTaq® Enviro Wastewater SARS-CoV-2 System, E/N2	200 reactions/kit	E, N2 and PMMoV	N+E and PMMoV RNA	IAC and PMMoV
AM2110	GoTaq® Enviro Wastewater SARS-CoV-2 System, N1	200 reactions/kit	N1 and PMMoV	N+E and PMMoV RNA	IAC and PMMoV
AM2120	GoTaq® Enviro Wastewater SARS-CoV-2 System, N2	200 reactions/kit	N2 and PMMoV	N+E and PMMoV RNA	IAC and PMMoV
AM2130	GoTaq® Enviro Wastewater SARS-CoV-2 System, E	200 reactions/kit	E and PMMoV	N+E and PMMoV RNA	IAC and PMMoV
AM2100	GoTaq® Enviro Wastewater SARS-CoV-2 System, N1/N2/E	200 reactions/kit	N1, N2 or E and PMMoV	N+E and PMMoV RNA	IAC and PMMoV
AM2140	GoTaq® Enviro PMMoV Quant Kit, Quasar® 670	100 reactions/kit	PMMoV	PMMoV RNA
AM2050	SARS-CoV-2 (N+E) RNA	100µl		SARS-CoV-2 RNA
AM2060	SARS-CoV-2 (N+E) dsDNA	100µl		SARS-CoV-2 DNA
AM2070	PMMoV RNA	100µl		PMMoV RNA
CS317419	GoTaq® Enviro HF183/crAssphage Quant Kit	100 reactions/kit	crAssphage and HF183	DNA	IPC
CS317420	GoTaq® Enviro Noro/HepA RT-qPCR System	100 reactions/kit	Norovirus GI, Norovirus GII and Hepatitis A Virus	DNA
CS317421	GoTaq® Enviro FluA/FluB/SARS-CoV-2/RSV	100 reactions/kit	Influenza A, Influenza B, SARS-CoV-2 “SC2” and RSV (A+B)	DNA
CS317423	GoTaq® Enviro Candida auris qPCR System	100 reactions/kit	Candida auris FSK1 and HF183	DNA
CS346201	ZDC	200 reactions/kit	Zika Virus, Chikungunya Virus, Dengue Virus	Available separately upon request	RNase P
CS317417	MS2 RT-qPCR Kit	100 reactions/kit	MS2	MS2 DNA

How Wastewater Testing Can Help Campuses Reopen

Robert Brooks is the Technical Director and Operations Manager at Microbac Laboratories in Oak Ridge, Tennessee. Since the start of the pandemic, his lab has begun developing methods for environmental testing of the SARS-CoV-2 virus using PCR analysis. They based their PCR tests on primer sets used for clinical testing recommended by the CDC, but they had to add other components. “You can’t just take a clinical test and start running environmental samples. It doesn’t quite work like that.”, says Robert. The internal controls in clinical testing kits were meant for human samples, not wastewater. To ensure accurate results, they needed a kit specifically designed for wastewater.

Learn more about how Microbac Laboratories is using the SARS-CoV-2 RT-qPCR Kit for Wastewater to develop low-cost SARS-CoV-2 surveillance testing for university campuses in this blog.

“The Promega kits include internal controls for all the different parameters that need to be measured. The separate targets give us a lot of flexibility, which is what we need."

Robert Brooks, Technical Director and Operations Manager, Microbac Laboratories

Protocols

Complete Protocol

GoTaq® Enviro Wastewater SARS-CoV-2 Systems Technical ManualPDF (1527 KB) – English

GoTaq® Enviro PMMoV Quant Kit, Quasar® 670 Technical ManualPDF (1260 KB) – English

SARS-CoV-2 (N+E) RNA Quant Standard Product InformationPDF (114 KB) – English

SARS-CoV-2 (N+E) dsDNA Quant Standard Product InformationPDF (103 KB) – English

GoTaq® Enviro Wastewater SARS-CoV-2 Dual Target Systems Technical ManualPDF (1280 KB) – English

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
N1 and PMMoV Primer/Probe/IAC Mix, 20X	AM211A	1 × 100μl
N2 and PMMoV Primer/Probe/IAC Mix, 20X	AM212A	1 × 100μl
E and PMMoV Primer/Probe/IAC Mix, 20X	AM213A	1 × 100μl
Nuclease-Free Water	P119A-C	2 × 1.25ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
N1 and PMMoV Primer/Probe/IAC Mix, 20X	AM211A	2 × 100μl
Nuclease-Free Water	P119A-C	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
N2 and PMMoV Primer/Probe/IAC Mix, 20X	AM212A	2 × 100μl
Nuclease-Free Water	P119A-C	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
E and PMMoV Primer/Probe/IAC Mix, 20X	AM213A	2 × 100μl
Nuclease-Free Water	P119A-C	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

What's in the box?

Item	Part #	Size	Available Separately
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	1 × 1ml
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
PMMoV Primer/Probe Mix, Quasar® 670	AM214A	1 × 100μl
CXR Reference Dye	C541A	1 × 100μl	View Product
Nuclease-Free Water	P119A-C	1 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

What's in the box?

Item	Part #	Size
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size
SARS-CoV-2 (N+E) dsDNA, 2 × 10⁶ copies/µl	AM206A	1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
N1/N2 & PMMoV Primer/Probe/IAC Mix, 20X	AM215A	2 × 100μl
Nuclease-Free Water	P119A-C	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

What's in the box?

Item	Part #	Size
GoScript™ Enzyme Mix	A276A	1 × 100μl
GoTaq® Enviro Master Mix, 2X	AM200A	2 × 1ml
SARS-CoV-2 (N+E) RNA, 4 × 10⁶ copies/µl	AM205A	1 × 100μl
PMMoV RNA, 4 × 10⁶ copies/µl	AM207A	1 × 100μl
E/N2 & PMMoV Primer/Probe/IAC Mix, 20X	AM216A	2 × 100μl
Nuclease-Free Water	P119A-C	2 × 1.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

SARS-CoV-2 RT-qPCR Kits for Wastewater

Convenient Kits for Quantifying SARS-CoV-2 in Wastewater

Sensitive Quantification of SARS-CoV-2 Viral RNA in Wastewater

Select Which Product is Right for You

How Wastewater Testing Can Help Campuses Reopen

Protocols

Complete Protocol

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Resources

Articles

Other Resources

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