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GloMax® Discover Microplate Reader

Multimode Microplate Reader with Advanced Detection Capabilities

  • Luminescence, fluorescence, UV-visible absorbance, BRET and FRET detection
  • 6- to 384-well microplate reader
  • Optimized with Promega cell viability and genetic reporter assays


Catalog number selected: GM3000

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GloMax® Discover Microplate Reader
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Easy-to-Use Plate Reader 

GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance.

  • High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities
  • Can be used as a standalone plate reader or integrated into high-throughput automated workflows
  • Integrated data analysis software makes results easy to interpret
Glomax plate reader for Luminescence Detection

Preloaded Protocols for Faster, Easier Data Analysis 

GloMax® plate readers have high sensitivity and a wide dynamic range, so you can detect both high and low signals on the same plate and have confidence in the accuracy of the signal recorded from each well. These instruments provide a range of options for your throughput needs and are equipped with integrated software that makes data analysis easy. GloMax® instruments come preloaded with protocols designed to work seamlessly with a wide variety of Promega luminescent and fluorescence-based assays at the touch of a button.
Compatible Promega Assays

GloMax® Instruments Come with Dozens of Preloaded Protocols

glomax navigator has 40+ preloaded protocols
glomax explorer has 65+ preloaded protocols
glomax discover has 70+ preloaded protocols

GloMax® Application Notes

No need to reinvent the wheel—we’ve already used GloMax® instruments for everything from cell viability, toxicity and metabolism, kinase and cell signaling, reporter gene assays, nucleic acid quantitation for NGS sequencing, PROTACs and protein degradation, bioassays, BRET, ELISAs and even water quality testing. Search our Application Notes collection for examples using GloMax® instruments with a wide range of bioluminescence- and fluorescence-based assays.

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GloMax Discover System Multimode Plate Reader

Microplate Reader Performance

GloMax® Discover multimode microplate reader delivers maximum flexibility with minimal user intervention and can be integrated into low- and medium-throughput automation workflows. The instrument provides superior luminescence sensitivity and dynamic range for both strong and weak bioluminescence experimental samples, as well as seamless integration with Promega bioluminescent cell and gene reporter assays.
GloMax Microplate Reader GM3000

  • Simple touch-screen navigation with full PC capabilities
  • Choose from over 50 preloaded protocols, and spend less time optimizing
  • Build custom protocols using the intuitive drag-and-drop interface
  • Export data to a network, cloud or any desired drive
Plate reader filter paddles for luminescence detection
Minimal Manual Intervention

  • Filter paddles and automatic filter switching allow easy two-color multiplexing assays or kinetic studies
  • Quick read-speeds for high efficiencies in your laboratory
  • Compatible for third-party automation control and data export to LIMS
GM3000 Luminescence Detection Plate Reader
Superior Sensitivity

  • Plate mask (aperture control) for switching between 96-well and 384-well plates
  • Low cross-talk between wells gives you better, more usable results
  • Broad dynamic range of the instrument extends the linear range of your assay
GloMax Microplate Reader integrates with robotics

  • Designed to comply with FDA 21CFR Part 11 regulations
  • Provides user authentication and authorization, data Integrity and protection, electronic signatures, and audit trails
  • IQ/OQ service available
  • Field-based service available

Accurate Results from Every Well

There are three ways that microplate readers can affect how much useful information you get from each plate. Poor sensitivity may cause you to miss low-level samples that could be the “hit” you are looking for. Letting the signal from bright wells spill into adjacent wells allows crosstalk to occur and skews your experimental results. And a narrow detection range limits the accuracy and reproducibility that your work requires. With the GloMax® plate readers, you can rest easy and have confidence in your results.
Illustration of GloMax plate reader sensitivity
Superior sensitivity so you don’t miss hits

GloMax® microplate readers offer superior sensitivity and auto-gain adjustment letting you measure weak and strong signals on the same plate.

GloMax plate readers minimize crosstalk
Masking so crosstalk doesn’t mislead your results

The masking design of GloMax® microplate readers means you can be confident that the results from each well are not skewed by signal from neighboring wells.

GloMax plate readers detect a broad signal range
Broad detection range so you don’t limit your experiments

With the broad detection range of GloMax® microplate readers, you can measure signals ranging from high intensity to near physiological levels.

Streamline Your Workflow with Automation

GloMax® Discover integrates with many commercially available automated systems, including liquid handers, plate stackers and robotic arms to enable seamless workflows for high-throughput assay quantitation and screening.

Our built-in and compliant SiLA2 communication driver makes integration easy.

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Microplate Reader Compliance for Your GxP Workflow


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Service and Support

One Call Supports It All

Promega supplies both the reagents and the instrument, so one call to Promega answers any questions you may have about assay chemistries or instrument performance.

Contact Technical Services

Ensure Minimal Instrument Downtime

  • Field Support and Loaner Programs
  • Service Packages
  • IQ and OQ Packages

Warranty and Service Agreements

  • Backed by a one-year warranty.
  • Additional warranty and service agreements are available.
  • For more information contact Promega Technical Services
Multiplate Reader & Fluorometer Support

Design Features

Instrument Specifications
Detection Modes Luminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET
Sample Format 6-, 12-, 24-, 48-, 96- and 384-well plates
Tablet PC Windows® 8.1 or 10 Tablet PC with USB connection to instrument
Read Speed 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes
Automation Compatibility Hardware and software accessible to third-party hardware/software control, including LIMS.
Dimensions 18.5in (46.9cm) width
19.5in (46.5cm) depth
10in (25.4cm) height without Tablet and PC mounted
16in (35.5cm) height with Tablet and PC mounted
Weight Approximately 60lb (27kg)
Power Requirements 120VA, 50/60HZ
Warranty 1-year parts and labor warranty included
Regulatory Compliance For Research Use Only. Not for use in Diagnostic Procedures
Luminescence Module Specifications
Detector Top-reading, head-on photon counting photomultiplier tube (PMT)
Wavelength Range 350–700nm
Detection Limit 3 × 10–21 moles of luciferase
Linear Dynamic Range 9 logs
Cross-talk Less than 3 × 10–5 (white, 96-well plate, Corning 3912)
Filtered Luminescence Built-in filter paddle
Fluorescence Module Specifications
Detector PIN-photodiode
Light Source Wavelength-matched LED
Read Position Top reading
Wavelengths selection Filter module with 5 standard excitation and emission filters. Empty filter positions are available for custom excitation and emission wavelengths.
Wavelengths included UV (Ex: 365nm, Em: 415–445nm) Blue (Ex: 475nm, Em: 500–550nm) Green (Ex: 520nm, Em: 580–640nm) Red (Ex: 627nm, Em: 660–720nm) AFC (Ex: 405nm, Em: 495–505nm)
Detection Limit 2fmol fluorescence dye/200µl
Linear Dynamic Range >6 logs (assay-dependent)
UV-Visible Absorbance Module Specifications
Detector Top-reading, head-on photon counting photomultiplier tube (PMT)
Light Source Xenon flash lamp
Spectra Range 200–600nm
Filter Wheel 9 absorbance filters included
Wavelengths included 230, 260, 280, 320, 405, 450, 490, 560 and 600nm 10nm band pass
Detection Limit 0.1 O.D.
Dynamic Range 0–4.0 O.D.
Injector System Specifications
Number of injectors (optional) Two injectors
Dispense Volume Range 5–200µl in 1µl increments
Plate Compatibility 6-, 12-, 24-, 48- and 96-well plates
Injection Speed 20–500µl per second
Waste Collection Tray Volume Approximately 50ml
Void Volume 500µl
Heating System Specifications
Temperature Range Ambient to 45°C
Shaker System Specifications
Shaking Pattern Linear or orbital patterns
Shaking Intensity 100–500 cycles/minute
PC Requirements
Operating System Windows® 8.1 or 10 Professional version, 64-bit, with USB connection to instrument
Disk Space 64GB
Other Requirements Microsoft.NET 4.6.1 Framework or newer (full version)

Available Separately

Product Catalog #
Luminometer Light Plate E6531
Bar Code Reader AS3200
USB Ethernet Adapter AS8403
GloMax® Discover Empty Luminescence Filter Paddle GM3011
GloMax® Discover Empty Fluorescence Filter Paddle GM3012
GloMax® Discover Luminescence Filter Paddle GM1090
GloMax® Discover Fluorescence Filter Paddle GM1100
GloMax® Discover 96 Half-Position Aperture Assembly GM1050
GloMax® Discover 96-Position Aperture Assembly GM3040
GloMax® Discover 384-Position Aperture Assembly GM3041
GloMax® Discover Aperture Plate GM3042
GloMax® Dual Injectors with Pumps GM3030
Inner and Outer Injector Tubing, Natural GM3014
GloMax® Explorer Absorbance Module Upgrade GM3520
GloMax® Discover Sales Rental E5301
GloMax® Discover or Explorer Installation Qualification SA1104
GloMax® Discover or Explorer Operational Qualification SA1105
GloMax® Discover or Explorer Installation and Operational Qualification SA1106
GloMax® Discover Standard Service Agreement SA4000
GloMax® Discover or Explorer Preventive Maintenance SA4030
GloMax® Discover Premier Service Agreement SA4002
GloMax® Discover Premier Warranty Upgrade SA4001


Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Patents and Disclaimers

European Pat. No. 2972400 and Japanese Pat. No. 6324485.

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