IdeS and IdeZ Protease are immunoglobulin G (IgG)-degrading enzymes that are valuable tools for the characterization of therapeutic antibodies, Fc fusion proteins and antibody-drug conjugates.
IdeS and IdeZ Proteases are fast, easy-to-use proteases; complete digestion can be achieved in 30 minutes without optimization. Cleavage is highly reproducible and specific, at a single site below the hinge region.
Both IdeS and IdeZ Proteases effectively cleave human IgG1, IgG2, IgG3 and IgG4, monkey, sheep, rabbit, humanized and chimeric IgGs as well as Fc-fusion proteins. However, only IdeZ Protease cleaves mouse IgG2a and IgG3.