Vascular Endothelial Growth Factor A (VEGF-A), a multifunctional cytokine, is important for normal development and the maintenance of the vascular and lymphatic systems. It is produced by many cells, including endothelial cells, macrophages and activated T cells. While VEGF-A plays a beneficial role in wound healing, its dysregulation contributes to tumorigenesis and vascular disease in various parts of the body.
The Lumit® VEGF-A Immunoassay quantitatively measures released VEGF-A in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer—the entire protocol is completed in less than 70 minutes. Use the assay directly on cells in culture or culture medium transferred to a separate assay plate.
Note: The total number of assays per kit will vary based on final well volume.
For standard well volumes:
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100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
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500 assay kits: applicable for 500 assays in 96-well plates or 2,000 assays in 384-well plates.
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1,000 assay kits: applicable for 1,000 assays in 96-well plates or 4,000 assays in 384-well plates.