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PowerPlex® Y23 System

5-Dye STR Multiplex for Amplification of 23 Y-STR Loci

  • High sensitivity in the presence of female DNA
  • High tolerance for inhibitors
  • Rapid-cycling protocols reduce amplification time by half

Size

Catalog number selected: DC2305

$ 1,751.00 Your price: Log in

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PowerPlex® Y23 System
50 reactions
$ 1,751.00
Your price: Log in
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Amplify 23 Y-STR Loci in a Single Reaction

The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples. Higher power of discrimination from 23 loci results in fewer false-positive matches and more meaningful STR analysis. A single kit for both casework and databasing simplifies your workflow.

The PowerPlex® Y23 System allows co-amplification and four-color detection of 23 male-specific STR loci: DYS576, DYS389I/II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438 (penta), DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643 (penta), DYS393, DYS458, DYS385a/b, DYS456 and Y-GATA-H4.

ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

Amplify Only Male DNA

The PowerPlex® Y23 System can successfully amplify trace amounts of male DNA, even in the presence of overabundant amounts of female DNA.

13065MA-W
Amplification of decreasing amounts of male DNA in the presence of 1µg of female DNA with two Y-STR kits: PowerPlex® Y23 System and Yfiler® Plus PCR Amplification Kit. Amplified products were separated on an Applied Biosystems® 3500xL Genetic Analyzer (1.2kV, 24-second injection).

Obtain Full Profiles from the Most Challenging Samples

The PowerPlex® Y23 System is a robust multiplex that is tolerant of many amplification inhibitors, including hematin, tannic acid and humic acid. The robust performance of the kit results in more interpretable data and less need for re-amplification of inhibitor-laden samples.

13066MA-W
Amplification of 500pg of male DNA (PowerPlex® Y23 System) or 1.0ng of male DNA (Yfiler® Plus PCR Amplification Kit) in the presence of increasing amounts of common inhibitors. DNA was amplified for 30 cycles, and amplified products were separated on an Applied Biosystems® 3500xL Genetic Analyzer (1.2kV, 24-second injection).

Streamline Workflows with Direct Amplification

The PowerPlex® Y23 System also is compatible with direct amplification, enabling streamlined Y-STR databasing efforts. Amplification can be successfully performed with sample types such as FTA® card punches as well as pretreated swabs, Bode Buccal DNA Collector™ punches or S&S 903 punches. Faster cycling conditions cut amplification time almost in half. Moreover, reduced sample-processing time and faster cycling conditions provide a significant time savings in every run.

Buccal Sample on FTA® Card
10433MA-W
Amplification of DNA from two 1.2mm punches from buccal cells on an FTA® card (direct amplification) using 27 cycles and the PowerPlex® Y23 System. Amplified products were separated using an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
Swab Sample with SwabSolution™ Reagent
13067MA-W
Amplification of DNA from a cotton swab pretreated with SwabSolution™ Reagent using 26 cycles and the PowerPlex® Y23 System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
Buccal Sample with PunchSolution™ Reagent
13068MA-W
Amplification of one 1.2mm Bode Buccal DNA Collector™ punch preprocessed with the PunchSolution™ Reagent using 26 cycles and the PowerPlex® Y23 System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Rapid Processing Solutions for PowerPlex®

The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.

Which Product is Right for Me?

ID_Automation

Want to Automate?

Want to spend more time on STR data analysis? Let the STR Normalization Manager Software set up your PCR amplifications using the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan) or MicroLab STAR (Hamilton) liquid handlers. The software uses quantitation data from the PowerQuant® or Plexor® HY Systems to accurately dilute and set up STR amplification of extracted DNA. The software can also set up direct amplification from swabs and card punches.

Automate Your STR Setup

Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.

Learn more
Consumables
Product Size Cat. #
1.1ml, Square-Well V-bottom Deep Well Plate
25/pack
2.2ml, Square-Well Deep Well Plate
50/pack
Water, Amplification Grade
6,250ml
Hardware
Product Cat. #
Four-Position Tube Holder
Heat Block Adapter
Plate Clamp 96
Plate Stand
Software
Product Cat. #
STR Normalization Manager Software

Specifications

You are viewing: DC2305 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product
PowerPlex® Y23 5X Master Mix DP108A 1 × 250μl

Water, Amplification Grade

DW099A 1 × 1,250μl View Product
PowerPlex® Y23 Allelic Ladder Mix DG248B 1 × 25μl

WEN Internal Lane Standard 500 Y23

DG520A 1 × 200μl View Product
PowerPlex® Y23 10X Primer Pair Mix DK209B 1 × 125μl

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

TMR-ET, CXR-ET and WEN dyes are proprietary.

Specifications

You are viewing: DC2320 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

Water, Amplification Grade

DW099A 5 × 1,250μl View Product
PowerPlex® Y23 Allelic Ladder Mix DG248B 4 × 25μl

WEN Internal Lane Standard 500 Y23

DG520A 1 × 200μl View Product
PowerPlex® Y23 10X Primer Pair Mix DK209B 4 × 125μl
PowerPlex® Y23 5X Master Mix DP108A 4 × 250μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

TMR-ET, CXR-ET and WEN dyes are proprietary.

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