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MTase-Glo™ Methyltransferase Assay

Measure Methyltransferase Activity Using a Universal Bioluminescent Assay

  • Simple add-and-read format
  • Use with any methyltransferase
  • No need to modify substrates

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Catalog number selected: V7601

$ 505.00
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MTase-Glo™ Methyltransferase Assay
400 assays
$ 505.00
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32463455-L1-MTase-Glo-Overview-Background

Measure Methyltransferase Activity Using a Universal Bioluminescent Assay

The MTase-Glo™ Methyltransferase Assay is a novel, bioluminescence-based assay that can be used to monitor the activities of methyltransferases (MTases) and their modulation by small molecules in many applications, including high-throughput screening.

Monitors formation of the reaction product S-adenosyl homocysteine (SAH) and can detect changes in activity of a broad range of methyltransferases, including DNA, protein, RNA and small molecule methyltransferases.

Used successfully for all classes of protein methyltransferases (lysine and arginine) and with different types of substrates (peptides, large proteins and even nucleosomes) to determine the specificity of these enzymes and their substrate requirements.

32463455-3D-Histones2

Universal Assay

An Overview of the MTase-Glo™ Methyltransferase Assay

13146MA-W

Measure the Activity of a Wide Array of Methyltransferases

Whether you are interested in protein, DNA, RNA or small molecule methyltransferases, the MTase-Glo™ Methyltransferase Assay can measure the activity of them all based on the reaction product S-adenosyl homocysteine (SAH). After the methyltransferase reaction is complete, just add the MTase-Glo™ Reagent and MTase-Glo™ Detection Solution to convert SAH to ATP and measure the light from the luciferase reaction.

Luminescence is measured using a plate-reading luminometer and can be correlated to SAH concentration using an SAH standard curve. The half-life of the luminescent signal is greater than 4 hours. This extended signal half-life eliminates the need for luminometers with injectors and allows batch-mode processing of multiple plates.

Determine the Optimal Enzyme Concentration

Titration of Methyltransferases Using the MTase-Glo™ Assay

13995MA-W
PRMT5/MEP50, a histone H3 methyltransferase
13996MA-W
EHMT2-G9a, a histone H3 methyltransferase
13997MA-W
DNMT3a, a DNA methyltransferase
13998MA-W
DOT1L, a histone H3 methyltransferase

Sensitive Detection

Luminescent Assay Offers Low Background and Wide Dynamic Range

The MTase-Glo™ Methyltransferase Assay produces excellent signal-to-noise ratios at low enzyme concentrations. Use the assay with a broad range of substrates, with no limitations on using high substrate concentrations or substrate type (short versus long peptides), to generate kinetic data and determine the mechanism of action of various methyltransferase modulators. There is also no need to modify substrates, which can lead to kinetic artifacts. Optimized for screening applications, the MTase-Glo™ Assay is not susceptible to false hits from fluorescence interference.

High-concentration SAH (up to 10µM)

13999MA-W

Low-concentration SAH (up to 1µM)

14000MA-W

Specifications

You are viewing: V7601 Change Configuration

What's in the box?

Item Part # Size

SAM, 1mM

A120B 1 × 200μl

SAH, 15µM

A233B 1 × 300μl

MTase-Glo™ Reagent, 10X

V760A 1 × 1ml

MTase-Glo™ Detection Solution

V761A 1 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: V7602 Change Configuration

What's in the box?

Item Part # Size

SAM, 1mM

A120C 1 × 1ml

SAH, 15µM

A233B 1 × 300μl

MTase-Glo™ Reagent, 10X

V760B 1 × 5ml

MTase-Glo™ Detection Solution

V761B 1 × 50ml

SDS

Choose language:

Certificate of Analysis

See all certificates for V7602 Search
View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

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