We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Have you purchased from Promega in the last six months? Take our brief survey ›

NanoBRET™ Target Engagement Assay Components

For Use With All NanoBRET™ TE Assays

  • DNA Bundles and Transfection Carrier DNA
  • Intracellular TE Nano-Glo® Substrate/Inhibitor
  • Tracer Dilution Buffer

Choose an option

Size

Catalog number selected: N2150

$ 2,754.00
Your price:
Add to Cart
This product is discontinued
NanoBRET™ Target Engagement Assay Components
BET BRD DNA Bundle/1 each
$ 2,754.00
Your price: Log in
Change Configuration
32463142-NanoBretBETBRD-OverviewImage2

NanoBRET™ Technology for Target Engagement

The NanoBRET™ Target Engagement (TE) Assay measures compound binding at select target proteins in intact cells, in real time. The NanoBRET™ TE Assay uses four key components: An expressed cellular target protein that is fused to the bright NanoLuc® luciferase; a cell-permeable fluorescent tracer that specifically binds to the target protein; a substrate for NanoLuc® luciferase; and a cell-impermeable inhibitor for NanoLuc® luciferase. 

The assay uses bioluminescence resonance energy transfer (BRET), achieved by transferring the luminescent energy from NanoLuc® luciferase to the fluorescent tracer that is bound to the target protein-NanoLuc® fusion. This energy transfer makes it possible to directly measure compound binding affinity as well as compound-target residence time.

Compounds that are applied to the cells and specifically engage the intracellular target protein-NanoLuc® fusion will result in a decrease in BRET. To ensure accurate assessment of intracellular target engagement, a NanoLuc® inhibitor is used to mitigate any extracellular NanoLuc® signal that may arise from cells compromised during handling, while not adversely affecting NanoLuc® luciferase expressed within healthy living cells

DNA Bundle Components

HDAC NanoLuc® Fusion Vectors

13726MA-W
HDAC2 NanoLuc FL Fusion Vector
HDAC3 NanoLuc FL Fusion Vector
NanoLuc HDAC6 FL Fusion Vector
NanoLuc HDAC6 CD2 Fusion Vector
HDAC10 NanoLuc Fusion Vector

BET BRD NanoLuc® Fusion Vectors

13738MA-W
13739MA-W
HDAC10 NanoLuc Fusion Vector
13740MA-W
13741MA-W
13742MA-W
13743MA-W
12900MA-W

Specifications

You are viewing: N2150 Change Configuration

What's in the box?

Item Part # Size

NanoLuc®-BRD4 FL Fusion Vector

N169A 1 × 20μg

NanoLuc®-BRD4 BD1 Fusion Vector

N170A 1 × 20μg

NanoLuc®-BRD3 FL Fusion Vector

N226A 1 × 20μg

NanoLuc®-BRDT FL Fusion Vector

N227A 1 × 20μg

NanoLuc®-BRD2 FL Fusion Vector

N228A 1 × 20μg

NanoLuc®-BRD2 BD1 Fusion Vector

N229A 1 × 20μg

NanoLuc®-BRD2 BD2 Fusion Vector

N230A 1 × 20μg

NanoLuc®-BRD4 BD2 Fusion Vector

N232A 1 × 20μg

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for N2150

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product (Nano-Glo® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730 and 9,951,373 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862 and other patents and patents pending.

U.S. Pat. Nos. 8,809,529 and 9,139,836, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,056,885 and 10,139,400, European Pat. No. 2782916 and other patents pending.

Licensed under EP1295121 and EP1088233.

Specifications

You are viewing: N2120 Change Configuration

What's in the box?

Item Part # Size

HDAC1-NanoLuc® FL Fusion Vector

N220A 1 × 20μg

HDAC2-NanoLuc® FL Fusion Vector

N221A 1 × 20μg

HDAC3-NanoLuc® FL Fusion Vector

N222A 1 × 20μg

NanoLuc®-HDAC6 FL Fusion Vector

N223A 1 × 20μg

NanoLuc®-HDAC6 CD2 Fusion Vector

N224A 1 × 20μg

HDAC10-NanoLuc® FL Fusion Vector

N225A 1 × 20μg

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for N2120

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product (Nano-Glo® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730 and 9,951,373 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862 and other patents and patents pending.

U.S. Pat. Nos. 8,809,529 and 9,139,836, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,056,885 and 10,139,400, European Pat. No. 2782916 and other patents pending.

Licensed under EP1295121 and EP1088233.

Specifications

You are viewing: N2161 Change Configuration

What's in the box?

Item Part # Size

NanoBRET™ Nano-Glo® Substrate

N157D 1 × 3.3ml

Extracellular NanoLuc® Inhibitor

N235C 1 × 1.1ml

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for N2161

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(b) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,809,529 and 9,139,836, European Pat. No. 2635582 and other patents and patents pending.

Patent Pending.

Licensed under EP1295121 and EP1088233.

Specifications

You are viewing: N2160 Change Configuration

What's in the box?

Item Part # Size

NanoBRET™ Nano-Glo® Substrate

N157C 1 × 330μl

Extracellular NanoLuc® Inhibitor

N235B 1 × 110μl

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for N2160

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(b) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,809,529 and 9,139,836, European Pat. No. 2635582 and other patents and patents pending.

Patent Pending.

Licensed under EP1295121 and EP1088233.

Specifications

You are viewing: N2191 Change Configuration

What's in the box?

Item Part # Size

Tracer Dilution Buffer

N219B 1 × 50ml

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for N2191

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Specifications

You are viewing: E4881 Change Configuration

What's in the box?

Item Part # SizeConcentration

Transfection Carrier DNA

E488A 5 × 20μg1μg/μl

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for E4881

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Let's find the product that meets your needs.

Talk to a Scientist

Petra

Petra

Germany