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HDAC-Glo™ Class IIa and HDAC-Glo™ 2 Assays

Detects HDAC Class IIa or Class I Enzyme 2 Activity

  • Single-reagent-addition homogeneous, luminescent assays
  • Measures the relative activity of histone deacetylase (HDAC) Class IIa and Class I enzyme 2
  • Use cells, extracts or purified enzymes

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Catalog number selected: G9560

$ 485.00
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HDAC-Glo™ Class IIa and HDAC-Glo™ 2 Assays
HDAC-Glo™ Class IIa/10ml
$ 485.00
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Predictive, Flexible Assays for HDAC Activity

The HDAC-Glo™ Class IIa and HDAC-Glo™ 2 Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class IIa and Class I enzyme 2, respectively, in the same, convenient platform using purified enzymes, extracts or cells directly in culture plates. The assays can be used to determine HDAC inhibitor potency, selectively profile HDAC inhibitors, correlate HDAC inhibitor potency with cellular fate in same-well multiplexed viability assays or determine off-target HDAC effects of compounds.

The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the deacetylated peptide from the aminoluciferin substrate, releasing aminoluciferin, which is quantified in a reaction using Ultra-Glo™ Recombinant Luciferase (firefly). This technology also allows you to multiplex with cell-health assays, offering more biologically relevant data within a predictive, cell-based context.

Provide Relevant Insight into Compound Effects in Biological Setting:
Make better decisions about your compound library early in drug screening.

Panel of Screening Tools Allows Comprehensive Screening of HDAC Activity:
Easy detection of Class IIa or Isozyme 2 in the same, convenient platform.

Highly Sensitive:
Feel confident because you can see more. Obtain a dynamic range 10- to 100-fold higher than comparable fluorescence methods.

Flexible Format:
Determine inhibitor performance in both biochemical and predictive cell-based formats using viable cells or in vitro with cell extracts or purified recombinant enzymes.

Simple Measurement of Deacetylating Activities:
Easy implementation from benchtop to screening with a single-reagent-addition, homogeneous, add-mix-measure protocol.

Fast Data Acquisition:
Achieve maximum signal in as little as 15 minutes with persistent glow-type steady-state signal, making the protocol amenable to automation in high-throughput formats and compatible with luminometers without injectors.

Robust Detection:
Minimize assay interference often encountered with fluorescent assays with robust, bioluminescence-based detection. This technology also allows you to multiplex with cell-health assays, offering more biologically relevant data within a predictive, cell-based context.

Number of Assays Per Plate

Product Cat.# # Assays Volume/Assay Plate Size
HDAC-Glo™ Class IIa Assay G9560 100 100µl 96-well
500 20µl 384-well
1,000 10µl 384-well
HDAC-Glo™ 2 Assay G9590 100 100µl 96-well
500 20µl 384-well
1,000 10µl 384-well

Specifications

You are viewing: G9560 Change Configuration

What's in the box?

Item Part # SizeConcentration

Developer Reagent

G653A 1 × 10μl

HDAC-Glo™ Class IIa Buffer

G954A 1 × 25ml

HDAC-Glo™ IIa Substrate

G955A 1 × 70μl10mM

Luciferin Detection Reagent

V859A 1 × 1 each

SDS

Choose language:

Download SDSPDF (182 KB) – English (United States)

Certificate of Analysis

See all certificates for G9560

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. No. 8,632,992 and other patents pending.

Promega has a non-exclusive, worldwide license to U.S. Pat. Nos. 7,033,778, 7,256,013, Europe Pat. No. 1243568 and Japan Pat. No. 4267043 and related applications to manufacture, have manufactured, use, possess, distribute, market, sell, offer for sale, and import deacetylase activity assay kits and related products for research and laboratory use (including the use on human derived cell lines, but excluding such use on human subjects for diagnostic or therapeutic purposes), product control, process control, product development and process development.

Furthermore, the rights above are extended to purchasers of licensed products from Promega to use, possess, distribute, market and sell such licensed products for the same purposes and for the same scope as granted to Promega.

Specifications

You are viewing: G9590 Change Configuration

What's in the box?

Item Part # SizeConcentration

Developer Reagent

G653A 1 × 10μl

HDAC-Glo™ 2 Buffer

G957A 1 × 25ml

HDAC-Glo™ 2 Substrate

G958A 1 × 10μl10mM

Luciferin Detection Reagent

V859A 1 × 1 each

SDS

Choose language:

Download SDSPDF (182 KB) – English (United States)

Certificate of Analysis

See all certificates for G9590

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. No. 8,632,992 and other patents pending.

Promega has a non-exclusive, worldwide license to U.S. Pat. Nos. 7,033,778, 7,256,013, Europe Pat. No. 1243568 and Japan Pat. No. 4267043 and related applications to manufacture, have manufactured, use, possess, distribute, market, sell, offer for sale, and import deacetylase activity assay kits and related products for research and laboratory use (including the use on human derived cell lines, but excluding such use on human subjects for diagnostic or therapeutic purposes), product control, process control, product development and process development.

Furthermore, the rights above are extended to purchasers of licensed products from Promega to use, possess, distribute, market and sell such licensed products for the same purposes and for the same scope as granted to Promega.

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