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QuantiFluor® dsDNA System

E4870

Sensitive Quantitation of Double-Stranded DNA in Solution

  • Specific quantitation of dsDNA
  • More sensitive than NanoDrop for low-concentration samples

Size

Catalog number selected: E2670

$ 260.00 Your price: Log In

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QuantiFluor® dsDNA System
1ml
$ 260.00
Your price: Log In
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Simple, Accurate DNA Quantitation. No Dilutions. No extra Tubes

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important. This dye shows minimal binding to single stranded DNA (ssDNA), RNA, protein and interfering compounds, allowing specific quantitation of dsDNA.

32462947-dsDNA-overview

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

The Quantifluor dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. 10pg/µl vs. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume).

12114MC-W
Sample*Assay

Quantifluor® dsDNA System

0.01–200ng/µl
0.01–200ng
Quant-iT™ PicoGreen
0.02–200ng/µl
0.02–200ng
Qubit® dsDNA HS
0.2–100ng/µl
0.2–100ng
NanoDrop® 2000 Spectrophotometer
2–12,000ng/µl
2–12,000ng

*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.


Better Sensitivity than Quant-iT™ PicoGreen®, Equivalent Linearity

The QuantiFluor® dsDNA System detects as little as 50pg/ml dsDNA in a 96-well microplate assay (1µl of a 10ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

9587ME-W-2
QuantiFluor dsDNA - Secondary Background Image

Proven for DNA Quantitation in Next-Gen Sequencing

Many laboratories have published papers using QuantiFluor™ dyes in sequencing applications.

View Sample Citations

Easy Setup on Microplate or Single-Tube Fluorometers

System includes all the necessary reagents to quickly set up and quantitate dsDNA, and is easy to set up on microplate or single tube fluorometers, including the following Promega fluorometers.

Quantus-332x302

(Use Blue Channel Ex 46 nm, Em 515–575 nm)

GloMax-Multi-Jr-332x302

(With Cat #E6071 Fluorescence Optical Kit – BLUE; Ex 460 nm, Em 515–570 nm)


How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

32462609-chalktalk1-quantitation-uv-absorbance

Fluorescence vs qPCR

32462609-chalktalk2-quantitation-fluorescent-dye

Specifications

You are viewing: E2670 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

QuantiFluor® dsDNA Dye

E258A 1 × 1ml

Lambda DNA Standard

E259A 1 × 100μg

20X TE Buffer (pH 7.5)

E260A 1 × 25ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

EE

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: E2671 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

QuantiFluor™ dsDNA Dye, 200X

E258B 1 × 100μl

Lambda DNA Standard

E259B 1 × 10μg

20X TE Buffer (pH 7.5)

E260B 1 × 5ml View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

EE

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

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