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T4 Polynucleotide Kinase

Transfers the γ-Phosphate from ATP to the 5´ End of Nucleotides

  • Can be inactivated by heating at 68°C for 10 minutes
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Labels the 5´ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting
  • Supplied at a concentration of 5–10u/μl

Size

Catalog number selected: M4101

$ 57.00 Your price: Log in

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T4 Polynucleotide Kinase
100u
$ 57.00
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T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation and cloning. T4 PNK is provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT.

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Storage Buffer: 20mM Tris-HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP and 50% (v/v) glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, DNase, RNase, endonuclease, end-labeling, blue/white cloning assay.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the 5´-OH end of a polynucleotide from [γ-32P]ATP in 30 minutes at 37°C. The reaction conditions are: 40mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl2, 5mM DTT, 0.1mM [γ-32P] ATP and 0.5mM 5´-OH polynucleotide end concentration.

Specifications

You are viewing: M4101 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Kinase 10X Buffer

C131B 1 × 100μl View Product

T4 Polynucleotide Kinase

M410A 1 × 100u

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: M4103 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Kinase 10X Buffer

C131A 2 × 500μl View Product

T4 Polynucleotide Kinase

M410B 1 × 1,000u5–10μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB
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