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SP6 RNA Polymerase

P1081_SP6-RNA-Polymerase--5-000u_3
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DNA-Dependent RNA Polymerase Specific for SP6 Promoter Sequences

  • Exhibits extremely high affinity and specificity for SP6 promoter sequences
  • >90% pure as determined by SDS polyacrylamide gel electrophoresis
  • Free of detectable levels of contaminating RNase and DNase activity (<1% release)

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Catalog number selected: P1085

$ 225.00
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SP6 RNA Polymerase
10–20u/µl/1,000u
$ 225.00
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SP6 RNA Polymerase is a DNA-dependent RNA polymerase used for in vitro transcription. Only SP6 DNA or DNA cloned downstream from an SP6 promoter can be used as a template for SP6 RNA Polymerase-directed RNA synthesis. The polymerase can incorporate 32P, 33P, 3H and 35S nucleoside triphosphates. SP6 RNA Polymerase is provided with 5X Reaction Buffer: Provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM spermidine, 50mM NaCl. The polymerase is offered as a high-concentration enzyme at 80u/µl (Cat.# P4084).

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Applications

  • Generation of templates for in vitro translation.
  • Generation of probes for nucleic acid hybridizations.
  • Generation of RNA processing substrates.
  • Generation of antisense RNA.

References

  1. Butler, E.T. and Chamberlin, M.J. (1982) J. Biol. Chem. 257, 5772–8.
  2. Melton, D.A. et al. (1984) Nucl. Acids Res. 12, 7035–56.

Storage Buffer: 20mM potassium phosphate buffer (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% (v/v) Triton® X-100 and 50% (v/v) glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100μl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2, 10mM DTT, 10mM NaCl, 2mM spermidine, 0.05% Tween® 20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5μCi [3H]CTP, and 2μg supercoiled pGEM®-5Zf(+) Vector DNA.

Specifications

You are viewing: P1085 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

SP6 RNA Polymerase

P108B 1 × 1,000u

DTT

P117B 1 × 250μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 × 500μl View Product

SDS

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Download SDSPDF (92 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: P1081 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

SP6 RNA Polymerase

P108G 1 × 5,000u

DTT

P117C 1 × 1.25ml100mM View Product

Transcription Optimized 5X Buffer

P118C 2 × 1.25ml View Product

SDS

Choose language:

Download SDSPDF (92 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: P4084 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

DTT

P117B 1 × 250μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 × 500μl View Product

SP6 RNA Polymerase (HC)

P408A 1 × 2,500u

SDS

Choose language:

Download SDSPDF (244 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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